2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

Pāpaʻa Enzyme: Hoʻohana mau ʻia ʻo ONPG ma ke ʻano he pani no beta-galactosidase, he enzyme e hydrolyzes ONPG e hana i kahi pūhui kala melemele (o-nitrophenol) hiki ke ʻike maʻalahi i ka spectrophotometrically.Hiki ke hoʻohana ʻia kēia hopena enzymatic e ana i ka hana o ka beta-galactosidase, e hana ana i ka ONPG i mea waiwai nui i nā haʻawina enzymology.

Nā ho'āʻo biology molecular: Hoʻohana ʻia ʻo ONPG ma ke ʻano he substrate i nā ʻano hoʻokolohua molecular biology, ʻoi aku ka nui o ka beta-galactosidase reporter gene assays.Ma kēia mau ho'āʻo, hoʻohana ʻia kahi substrate e pili ana i ka ONPG e ana i ka hana o ka gene reporter, ka mea maʻamau i hoʻoponopono ʻia e kekahi ʻano mea hoʻolaha o ka hoihoi.ʻO ka hana beta-galactosidase, i hōʻike ʻia e ka hoʻololi kala i hana ʻia ma ONPG hydrolysis, hāʻawi i ka ʻike waiwai e pili ana i ka hana hoʻolaha.

Ka nānā 'ana i ka hua'ōlelo Gene: Ho'ohana 'ia 'o ONPG i ka ho'opili 'ana i ka hō'ike gene.Ma ka hoʻopili ʻana i ke kaʻina hoʻolaha o ka hoihoi i ka gene beta-galactosidase, hiki i nā mea noiʻi ke ana i ka hana beta-galactosidase me ka hoʻohana ʻana iā ONPG ma ke ʻano he substrate.ʻO ke kiʻekiʻe o ka hana beta-galactosidase e hōʻike ana i ka ikaika a me ka hana o ka mea hoʻolaha, e ʻae ana i ka loiloi o nā pae hōʻike gene.

Nā noi diagnostic: Hiki ke hoʻohana ʻia ka ONPG i nā noi diagnostic, ʻoi loa i ka ʻike ʻana i nā microorganism pathogenic.ʻO nā ʻano bacteria like ʻole, e like me Escherichia coli a me kekahi mau ʻano o Shigella a me Salmonella, hoʻopuka i ka beta-galactosidase hiki ke hoʻokaʻawale iā ONPG.ʻO kēia hopena hydrolysis e hoʻololi i ka waihoʻoluʻu ʻike ʻia, hiki ke hoʻohana ʻia no ka hoʻoholo ʻana i ka noho ʻana o kēia mau maʻi bacteria i nā laʻana lapaʻau.