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N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium paʻakai CAS:66992-27-6
ʻO N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium paʻakai, ʻike ʻia hoʻi ʻo HEPES sodium paʻakai, he mea hoʻohui kemika i hoʻohana mau ʻia ma ke ʻano he mea hoʻopaʻa pH i loko o nā laboratories biological a me kemika.Kōkua ia i ka mālama ʻana i kahi pae pH paʻa, e kūpono ia no nā noi e like me ka moʻomeheu cell, ka enzyme assays, protein study, electrophoresis, a me nā ʻano hana lāʻau.ʻO ka paʻakai sodium HEPES e hōʻoia i nā kūlana kūpono no nā kaʻina hana olaola a hoʻonui i ka pololei a me ka hilinaʻi o nā hopena hoʻokolohua.
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S-Butyrylthiocholine iodide CAS:1866-16-6
ʻO S-Butyrylthiocholine iodide he mea hoʻohui kemika i hoʻohana mau ʻia i nā hoʻokolohua biochemical a me nā enzymatic.He pani ia no ka enzyme butyrylcholinesterase (BChE) a hoʻohana ʻia e ana i kāna hana.
Ke hoʻokahe ʻia ʻo S-Butyrylthiocholine iodide e BChE, hana ia i ka thiocholine a me ka waikawa butyric e like me nā huahana.Hiki ke ana ʻia ka hoʻokuʻu ʻana o ka thiocholine me ka hoʻohana ʻana i kahi spectrophotometric a fluorometric assay, e ʻae ana i ka helu ʻana o ka hana BChE.
Hoʻohana pinepine ʻia ʻo S-Butyrylthiocholine iodide i nā hoʻonohonoho hoʻokolohua a me nā noiʻi e loiloi i ka hana o BChE i nā laʻana e like me ka plasma koko a i ʻole nā kiko.Hiki ke hoʻohana ʻia e loiloi i ka hana enzymatic o BChE a me kāna kuleana kūpono i nā kaʻina hana olaola like ʻole, a me ka nānā ʻana a me ka nānā ʻana i kekahi mau kūlana olakino.
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ABTS (2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) diammonium paʻakai) CAS:30931-67-0
ʻO ka Diammonium 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate), i kapa pinepine ʻia ʻo ABTS, he substrate chromogenic maʻamau i hoʻohana ʻia i nā hoʻokolohua biochemical, ʻoi aku ma ke kahua o ka enzymology.He pūhui synthetic ia i hoʻohana ʻia e ana i ka hana o nā enzymes like ʻole, me nā peroxidases a me nā oxidases.
ʻAʻole waihoʻoluʻu ʻo ABTS i kona ʻano hoʻoheheʻe ʻia akā huli i ka uliuli-'ōmaʻomaʻo ke hoʻoheheʻe ʻia e kahi enzyme i mua o ka hydrogen peroxide a i ʻole ka oxygen molecular.ʻO kēia hoʻololi kala ma muli o ka hoʻokumu ʻia ʻana o kahi cation radical, kahi e hoʻomoʻa ai i ka mālamalama i ka spectrum ʻike ʻia.
ʻO ka hopena ma waena o ABTS a me ka enzyme e hoʻopuka i kahi huahana kala i hiki ke ana spectrophotometrically.ʻO ka ikaika o ka waihoʻoluʻu e pili pono ana i ka hana enzymatic, e ʻae ana i nā mea noiʻi e loiloi nui i nā kinetics enzyme, inhibition enzyme, a i ʻole nā pilina enzyme-substrate.
Loaʻa i ka ABTS kahi ākea o nā noi ma nā ʻano ʻano like ʻole, me ka diagnostics clinical, pharmaceutical research, a me ka ʻepekema meaʻai.He koʻikoʻi koʻikoʻi a hāʻawi i kahi ākea ākea ākea, e lilo ia i koho kaulana no ka nui o nā hoʻokolohua biochemical.
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4-NITROPHENYLΑ-D-MALTOHEXAOSIDE CAS:74173-30-1
ʻO 4-Nitrophenyl α-D-maltohexaoside he hui ia no ka papa o nā loulou α-glycosidic.He mea ia o ka maltose, ʻo ia ka disaccharide i haku ʻia me ʻelua mau ʻāpana glucose.I loko o kēia pūhui, ua hoʻololi ʻia ka pūʻulu hydroxyl o ka ʻāpana glucose mua me kahi moiety nitrophenyl.
Hoʻohana mau ʻia kēia pūhui ma ke ʻano he substrate i nā hoʻokolohua enzymatic e aʻo ai i ka hana o nā enzyme like ʻole, ʻoi aku ka poʻe i pili i ka metabolism carbohydrate.Hiki i ka hui nitrophenyl ke ʻike maʻalahi a me ka helu ʻana i nā hopena enzymatic ma ke ana ʻana i ka absorbance a i ʻole fluorescence o ka huahana i ʻoki ʻia.
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PIPES CAS:5625-37-6 Kumukūʻai Mea Hana
ʻO PIPES (piperazine-1,4-bisethanesulfonic acid) he zwitterionic buffering pūhui i hoʻohana mau ʻia i ka noiʻi olaola a me ka biochemical.He paʻa pH maikaʻi ia me ka mana kiʻekiʻe no ka mālama ʻana i nā kūlana pH kūpaʻa i ka pae pH o 6.1 a 7.5.He liʻiliʻi ka hoʻopilikia ʻana o PIPES me nā biomolecules a kūpono ia no ka hoʻāʻo ʻana i ka wela.Hoʻohana pinepine ʻia i nā ʻenehana electrophoresis gel a me ka hoʻokumu ʻana i ka lāʻau lapaʻau ma ke ʻano he stabilizing agent.Ma ke ʻano holoʻokoʻa, ʻo PIPES kahi hui maʻalahi a hoʻohana nui ʻia i nā ʻano hoʻokolohua like ʻole.
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3,3′,5,5′-Tetramethylbenzidine CAS:207738-08-7
ʻO 3,3′,5,5′-Tetramethylbenzidine, ʻike ʻia hoʻi ʻo TMB, he mea hoʻohui kemika i hoʻohana mau ʻia ma ke ʻano he substrate chromogenic i nā enzyme-linked immunosorbent assays (ELISA) a me nā hoʻokolohua biochemical ʻē aʻe.Hoʻohana pinepine ʻia e ʻike a helu i ka hiki ʻana o nā enzymes e like me horseradish peroxidase (HRP) i loko o nā ʻano mea like ʻole.Hoʻololi ʻia ka TMB mai ke kala ʻole i ka uliuli i mua o kēia mau enzymes.A laila, hiki ke ho'ōki i ka pane ʻana ma ka hoʻohui ʻana i kahi waika e hoʻololi i ka waihoʻoluʻu polū i kahi kala melemele hope loa.ʻO ka ikaika o ka waihoʻoluʻu melemele e like me ka nui o ka enzyme i loaʻa, e ʻae ai i ka helu ʻana.
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APS-5 CAS:193884-53-6 Kumukūʻai Mea Hana
ʻO (4-Chlorophenyl) thio-methanol 1-(dihydrogen phosphate) disodium paʻakai (1:2) he mea hoʻohui kemika no ka papa o nā derivatives acridine.Loaʻa iā ia kahi ʻōnaehana apo 10-methylacridine me kahi hui thioether i hoʻopili ʻia i ke kūlana 4-chlorophenyl.Aia pū kekahi pūʻulu methanol a me ʻelua pūʻulu phosphate i hoʻokaʻawale ʻia e nā ion sodium.
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5-Bromo-4-chloro-3-indolyl phosphate disodium paʻakai CAS:102185-33-1
ʻO 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (BCIP) he mea hoʻohui kemika i hoʻohana mau ʻia i ka molecular biology a me nā noi biochemistry.He substrate chromogenic ia no nā enzymes alkaline phosphatase.
Hoʻohana pinepine ʻia ka BCIP me ka nitroblue tetrazolium (NBT) ma ke ʻano he substrate e ʻike ai i ka hana alkaline phosphatase.Ke hoʻoheheʻe ʻia ka BCIP e ka alkaline phosphatase, puka mai kahi ʻano polū polū, hiki ke ʻike i ke ʻano o ka enzyme a i ʻole ka hana.
Hoʻohana nui ʻia kēia pūhui i nā noi e like me ka immunohistochemistry, in situ hybridization, a me nā enzyme-linked immunosorbent assays (ELISAs) e ʻike ai i ka hele ʻana a i ʻole ka hoʻokaʻawale ʻana o nā biomolecules kikoʻī a i ʻole nucleic acids.Hāʻawi ka ʻeke polū i hoʻokumu ʻia e BCIP i kahi hōʻailona ʻike ʻia e kōkua ana i ka ʻike ʻana a me ka nānā ʻana o nā molekele i hoʻopaʻa ʻia i nā laʻana hoʻokolohua.
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Paʻakai Sodium CAS:139-41-3 Kumukūʻai Mea Hana
N,N-Bis(2-hydroxyethyl)glycine sodium paʻakai he mea hoʻohui kemika i hoʻohana ʻia ma ke ʻano he mea hoʻopaʻa i nā noi biochemical a me biophysical like ʻole.Kōkua ia i ka mālama ʻana i ka pae pH paʻa i nā kūlana hoʻokolohua, e hoʻohana pono ai i nā haʻawina enzyme, ka noiʻi protein, ka moʻomeheu cell, a me nā ʻenehana blotting Western.
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4-Aminophthalhydrazide AMPPD CAS:3682-14-2
ʻO 4-Aminophthalhydrazide, ʻike ʻia hoʻi ʻo 4-APhH, he mea hoʻohui kemika me ke ʻano molekala C8H8N2O.Aia ia i ka papa o nā pūhui hydrazide a loaʻa mai ka phthalic acid.
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N-Acetyl-L-cysteine CAS:616-91-1
ʻO ka N-Acetyl-L-cysteine (NAC) kahi ʻano hoʻololi o ka cysteine amino acid.Hāʻawi ia i kahi kumu o ka cysteine a hiki ke hoʻololi koke ʻia i ka tripeptide glutathione, kahi antioxidant ikaika i ke kino.ʻIke ʻia ʻo NAC no kāna mau antioxidant a me nā waiwai mucolytic, e hoʻohana pono ai i nā noi olakino.
Ma ke ʻano he antioxidant, kōkua ʻo NAC i ka pale ʻana i nā cell mai ka pōʻino i hoʻoiho ʻia e nā radical manuahi, nā ʻano oxygen reactive, a me nā toxins.Kākoʻo ia i ka glutathione synthesis, kahi mea koʻikoʻi i nā kaʻina detoxification o ke kino a me ka mālama ʻana i kahi ʻōnaehana olakino olakino.
Ua aʻo ʻia ʻo NAC no kāna mau pono kūpono i ke olakino hanu, ʻoi aku hoʻi no nā poʻe me nā kūlana e like me ka bronchitis maʻi, COPD, a me ka cystic fibrosis.Hoʻohana mau ʻia ʻo ia ma ke ʻano he expectorant e kōkua i ka hoʻokaʻawale ʻana a me ka hoʻokaʻawale ʻana i ka ʻōpala, e maʻalahi ai ka hoʻomaʻemaʻe ʻana i nā alaloa.
Eia kekahi, ua hōʻike ʻo NAC i ka ʻōlelo hoʻohiki i ke kākoʻo ʻana i ke olakino o ke ake ma ke kōkua ʻana i ka wehe ʻana i nā mea ʻona, e like me ka acetaminophen, kahi mea hoʻomaha ʻeha maʻamau.Loaʻa paha nā hopena pale i ka pōʻino o ka ate ma muli o ka inu ʻona.
Ma kahi o kāna mau antioxidant a me nā mea kākoʻo hanu, ua ʻimi ʻia ʻo NAC no kāna mau pono kūpono i ke olakino noʻonoʻo.Ke hōʻike nei kekahi mau noiʻi he hopena maikaʻi paha ia i nā maʻi ʻino, e like me ke kaumaha a me ka obsessive-compulsive disorder (OCD).
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Acetylthiocholine iodide CAS:1866-15-5
ʻO ka Acetylthiocholine iodide kahi hui kemika i hoʻohana mau ʻia ma ke ʻano he substrate i nā hoʻokolohua enzyme e ana i ka hana o ka enzyme acetylcholinesterase (AChE).ʻO AChE kahi enzyme e hydrolyzes i ka neurotransmitter acetylcholine, kahi hana koʻikoʻi i ka hoʻopau ʻana i ka hoʻouna ʻana i ka hōʻailona ma waena o nā pūnana nerve.
Ke hana ʻia ka acetylthiocholine iodide e AChE, hoʻoneʻe ʻia ka hui acetyl, ka hopena o ka hoʻokumu ʻana i nā ion thiocholine a me ka acetate.Hoʻopili ʻo Thiocholine me kahi reagent kala ʻole i kapa ʻia ʻo DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) e hana i kahi pūhui kala melemele i kapa ʻia ʻo 5-thio-2-nitrobenzoate, hiki ke ana spectrophotometrically.ʻO ka nui o ka hoʻomohala ʻana o ka waihoʻoluʻu pili pono i ka hana a AChE i ka hāpana.
