PIPES CAS:5625-37-6 Kumukūʻai Mea Hana

ʻO PIPES (piperazine-1,4-bisethanesulfonic acid) he zwitterionic buffering pūhui i hoʻohana mua ʻia i ka noiʻi biological a me biochemical.Loaʻa iā ia kekahi mau hiʻohiʻona nui a me nā noi, me:

ʻĀpana hoʻopaʻa pH: ʻO ka PIPES kahi pale kūpono e kōkua i ka mālama ʻana i kahi pae pH paʻa i nā hoʻokolohua ʻokoʻa like ʻole.Hoʻohana maʻamau ia i loko o ka media moʻomeheu cell, nā hoʻāʻo enzyme, a me nā noi biology molecular.

Hiki ke hoʻopaʻa haʻahaʻa kiʻekiʻe: Loaʻa i ka PIPES kahi mana hoʻopaʻa paʻa maikaʻi i loko o ka pae pH o 6.1 a 7.5, kūpono ia no ka mālama ʻana i nā kūlana pH kūpaʻa i kahi ākea o nā ʻōnaehana biological.

ʻO ka pilina liʻiliʻi me nā biomolecules: Ua ʻike ʻia ʻo PIPES no kona haʻahaʻa haʻahaʻa me nā kaʻina biochemical a me ka liʻiliʻi o ka hoʻopaʻa ʻana i nā proteins a me nā enzymes, e lilo ia i mea kūpono no ka mālama ʻana i ka pono a me ka hana o nā biomolecules.

He kūpono no nā ho'āʻo e pili ana i ka mahana: hiki iā PIPES ke mālama i kāna mau waiwai hoʻopaʻa ma luna o kahi ākea ākea, me ka physiological a me ka wela kiʻekiʻe.He kūpono kēia no nā hoʻokolohua e koi ana i ke kūpaʻa a me ka pololei i nā kūlana wela.

Nā noi Electrophoresis: Hoʻohana mau ʻia ʻo PIPES ma ke ʻano he pale i nā ʻenehana electrophoresis gel, e like me RNA a i ʻole DNA agarose gel electrophoresis, ma muli o kona haʻahaʻa haʻahaʻa UV a me nā waiwai conductivity kiʻekiʻe.

ʻO ka lāʻau lapaʻau: Hoʻohana pū ʻia ʻo PIPES i ka hoʻokumu ʻana i ka lāʻau lapaʻau ma ke ʻano he mea hoʻolalelale, e hāʻawi ana i ka paʻa a mālama i ka pH maikaʻi loa no ka pono o ka lāʻau.