PIPES sesquisodium paʻakai CAS:100037-69-2

Agent Buffering: Hoʻohana mau ʻia ʻo PIPES-Na3 ma ke ʻano he mea hoʻopaʻa i nā ʻano hoʻokolohua olaola a me ka biochemical.Kōkua ia i ka mālama ʻana i ka pH paʻa i ka pae i makemake ʻia, maʻamau ma waena o 6.1 a 7.5.

Culture Cell: Hoʻohana pinepine ʻia ʻo PIPES-Na3 ma ke ʻano he mea hoʻopaʻa i ka media moʻomeheu cell e mālama i ka pH o ka waena i ka wā o ka ulu ʻana o ke kelepona a me nā hoʻokolohua.Hāʻawi ia i kahi microenvironment paʻa no ka moʻomeheu cell a kōkua i ke kākoʻo ʻana i ka ola a me ka hana.

Nā Haʻawina Enzyme a me Protein: Hoʻohana nui ʻia ʻo PIPES-Na3 i nā haʻawina enzyme a me nā protein ma muli o kona hiki ke hoʻopaʻa i ka pae pH physiological.Kōkua ia i ka mālama ʻana i ka pH maikaʻi loa no ka hana enzyme, kūpaʻa, a me ke ʻano protein.

Gel Electrophoresis: PIPES-Na3 kūpono no ka hoʻohana ʻana ma ke ʻano he buffering agent i nā ʻenehana electrophoresis gel e like me SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis).Mālama ia i ka mālama ʻana i ka pH i makemake ʻia i loko o ka gel a me ke kaʻina kaʻawale.

Molecular Biology Techniques: Hoʻohana ʻia ʻo PIPES-Na3 i nā ʻenehana biology molekala like ʻole me ka RNA a me ka hoʻomaʻemaʻe DNA, PCR (Polymerase Chain Reaction), a me ka hoʻonohonoho DNA.Kōkua ia i ka mālama ʻana i ka pH i makemake ʻia a me ke kūpaʻa i kēia mau kaʻina hana.