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X-GAL CAS:7240-90-6 Kumukūʻai Mea Hana

ʻO ka 5-Bromo-4-chloro-3-indolyl-β-D-galactoside (X-Gal) kahi substrate chromogenic maʻamau i hoʻohana ʻia i ka molecular biology a me nā noi biochemistry.Hoʻohana nui ia no ka ʻike ʻana i ka gene lacZ, kahi e hoʻopili ai i ka enzyme β-galactosidase.


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Hoʻohana a me ka hopena

Hoʻololi kala: ʻAʻole waihoʻoluʻu ka X-Gal akā, ma ka hydrolysis e β-galactosidase, lilo ia i polū.Hiki i kēia hoʻololi kala ke ʻike maka a me ka helu ʻana o ka hana β-galactosidase.

LacZ Gene Detection: Hoʻohana ʻia ʻo X-Gal e ʻike i nā cell a i ʻole nā ​​hana genetic e hōʻike ana i ka gene lacZ.Hoʻohana maʻamau ʻia ʻo LacZ ma ke ʻano he moʻo nūhou i ka molecular biology e loiloi i ka hōʻike gene a i ʻole ke aʻo ʻana i ka hana hoʻolaha.

Nānā Koloni: Hoʻohana pinepine ʻia ʻo X-Gal i nā hoʻāʻo ʻana o ka colony bacterial.ʻIke ʻia nā ʻāpana bacteria e hōʻike ana i ka LacZ i ka uliuli ke ulu ʻia ma luna o ka agar i loaʻa iā X-Gal, e hiki ai ke ʻike maʻalahi a koho i nā ʻāpana lacZ-positive.

ʻIkepili Gene Fusion: Hoʻohana pū ʻia ʻo X-Gal i nā hoʻokolohua fusion gene.Ke hoʻopili ʻia kahi gene i hoʻopaʻa ʻia i ka gene lacZ, hiki i ka ʻili X-Gal ke hōʻike i ke ʻano hōʻike o ka protein fusion i loko o ke kelepona a i ʻole ka ʻiʻo.

Protein Localization: Hiki ke hoʻohana ʻia ka staining X-Gal e noiʻi i ka localization protein subcellular.Ma ka hoʻohui ʻana i kahi pūmua hoihoi i ka gene lacZ, hiki i ka hana β-galactosidase ke kuhikuhi i kahi o ka protein i loko o kahi keena.

X-Gal Analogues: Ua hoʻomohala ʻia nā ʻano hoʻololi o X-Gal, e like me Bluo-Gal a i ʻole Red-Gal, i mea e hiki ai i nā papa hana hoʻomohala kala ʻē aʻe.Hiki i kēia mau mea hoʻohālikelike ke hoʻokaʻawale i waena o nā cell lacZ-positive a me ka lacZ-negative a i ʻole nā ​​kikoʻī e hoʻohana ana i nā kala like ʻole.

Laʻana Huahana

7240-90-6-1
7240-90-6-2

Hoʻopili huahana:

6892-68-8-3

ʻIke hou aku:

Huina C14H15BrClNO6
Hoʻāʻo 99%
Ka nana aku Pauda keʻokeʻo
CAS No. 7240-90-6
Hoʻopili Liʻiliʻi a nui
Ola Pahu 2 makahiki
Waihona E mālama ma kahi maloʻo a maloʻo
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