2-NITROPHENYL-BETA-D-GLUCOPYRANOSIDE CAS:2816-24-2

ʻO ka substrate enzyme: Hoʻohana pinepine ʻia ʻo ONPG ma ke ʻano he substrate no ka beta-galactosidase, kahi enzyme e hydrolyzes iā ONPG e hana i kahi hui melemele (o-nitrophenol) hiki ke ʻike maʻalahi ʻia spectrophotometrically. Hiki ke hoʻohana ʻia kēia hopena enzymatic e ana i ka hana o ka beta-galactosidase, e hoʻolilo ana iā ONPG i mea hana waiwai nui i nā haʻawina enzymology.

Nā hoʻāʻo biology molekala: Hoʻohana ʻia ʻo ONPG ma ke ʻano he substrate i nā hoʻāʻo biology molekala like ʻole, ʻoi aku hoʻi i nā hoʻāʻo gene reporter beta-galactosidase. I loko o kēia mau hoʻāʻo, hoʻohana ʻia kahi substrate i hoʻokumu ʻia ma ONPG e ana i ka hana o ka gene reporter, ka mea i hoʻoponopono pinepine ʻia e kahi kaʻina hoʻolaha hoihoi. ʻO ka hana beta-galactosidase, i hōʻike ʻia e ka loli kala i hana ʻia ma luna o ka hydrolysis ONPG, hāʻawi i ka ʻike waiwai e pili ana i ka hana hoʻolaha.

Ka loiloi ʻana i ka hōʻike ʻana o ka gene: Hoʻohana ʻia ʻo ONPG i ka loiloi ʻana i ka hōʻike ʻana o ka gene. Ma ka hoʻopili ʻana i ke kaʻina hoʻolaha hoihoi i ka gene beta-galactosidase, hiki i nā mea noiʻi ke ana i ka hana beta-galactosidase me ka hoʻohana ʻana iā ONPG ma ke ʻano he substrate. Hōʻike ka pae o ka hana beta-galactosidase i ka ikaika a me ka hana o ka mea hoʻolaha, e ʻae ana i ka loiloi ʻana i nā pae hōʻike gene.

Nā noi diagnostics: Hiki ke hoʻohana ʻia ʻo ONPG i nā noi diagnostics, ʻoi aku hoʻi i ka ʻike ʻana i nā microorganism pathogenic. ʻO nā bacteria like ʻole, e like me Escherichia coli a me kekahi mau ʻano o Shigella lāua ʻo Salmonella, hana i ka beta-galactosidase hiki ke ʻoki i ka ONPG. Hoʻopuka kēia hopena hydrolysis i kahi loli kala i ʻike ʻia, hiki ke hoʻohana ʻia e hoʻoholo i ke alo o kēia mau bacteria i nā laʻana lapaʻau.