4-Nitrophenyl-2-acetamido-2-deoxy-β-D-glucopyranoside CAS:3459-18-5

ʻO ka Enzyme Substrate: Hoʻohana nui ʻia ka pNAG ma ke ʻano he substrate kikoʻī no nā enzymes like ʻole e pili ana i ka hydrolysis o nā pilina β-D-glucoside. Ke ʻoki kēia mau enzymes i ka molekala pNAG, hoʻokuʻu ia i ka p-nitrophenol. Hiki i kēia i nā mea noiʻi ke ana a helu i ka hana enzymatic.

Nā Hoʻāʻo Hana Enzyme: Hiki ke ʻike ʻia a ana ʻia ka hydrolysis o pNAG e nā enzymes kikoʻī ma ke ʻano spectrophotometrically. ʻO kēia ka mea e kūpono ai ka pNAG no nā hoʻāʻo hana enzyme, kahi e kūlike pololei ai ka nui o ka p-nitrophenol i hana ʻia me ka hana enzymatic.

Nānā ʻana i ka High-Throughput: Hoʻohana pinepine ʻia ka pNAG i nā hoʻāʻo nānā ʻana i ka high-throughput e ʻike a wehewehe i nā mea hoʻopaʻa enzyme a i ʻole nā ​​​​mea hoʻoulu. Ma ka loiloi ʻana i ka hopena o nā hui like ʻole ma ka hana enzymatic, hiki i nā mea noiʻi ke ʻike i nā moho lāʻau lapaʻau kūpono a i ʻole nā ​​​​mea hoʻololi o ka hana enzyme.

Nā Haʻawina Hōʻike Gene: Hoʻohana ʻia ka pNAG i ka noiʻi biology molecular e aʻo i ka hōʻike ʻana a me ka hoʻoponopono ʻana o ka gene. Ma ke ana ʻana i ka hana enzymatic o nā enzymes kikoʻī me ka hoʻohana ʻana i ka pNAG ma ke ʻano he substrate, hiki i nā mea noiʻi ke noiʻi i ka hopena o ka hōʻike ʻana o ka gene ma ka hana a me ka hana enzyme.