4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Ka hopena: ʻO ONPG kahi substrate i hoʻohana pono ʻia e ʻike i ke alo a me ka hana o ka enzyme β-galactosidase.Ke loaʻa ka enzyme β-galactosidase a hana, hoʻokaʻawale ʻo ia i ka ONPG i ʻelua mau huahana: o-nitrophenol a me kahi derivative galactose.ʻO ka hoʻokuʻu ʻana o ka o-nitrophenol ka hopena i kahi hoʻololi kala melemele, hiki ke ana ʻia me ka spectrophotometer.

Noi: He nui nā noi o ONPG i ka molecular biology a me ka noiʻi biochemistry:

Ka hoʻoholo ʻana o ka hana β-galactosidase: Hoʻohana mau ʻia ka ONPG e ana a helu i ka hana o ka enzyme β-galactosidase.Hiki ke ana ʻia ka nui o ka hoʻokumu ʻana o ka o-nitrophenol, i pili pono i ka hana enzyme, me ka spectrophotometrically.

Hōʻike Gene a me ka hoʻoponopono: Hoʻohana pinepine ʻia ʻo ONPG i nā hoʻokolohua e pili ana i ka hōʻike gene a me nā haʻawina hoʻoponopono.Hoʻohana pinepine ʻia ia i nā hoʻokolohua fusion protein, e like me ka ʻōnaehana lacZ fusion maʻamau, e aʻo i ka hōʻike ʻana o nā genes ma lalo o ka mana o nā mea hoʻolaha kikoʻī.Hāʻawi ka hana beta-galactosidase me ka hoʻohana ʻana i ka ONPG i nā ʻike i ke kiʻekiʻe o ka hōʻike gene.

Ka nānā ʻana no ka hana β-galactosidase: Hiki ke hoʻohana ʻia ka ONPG ma ke ʻano he ʻano nānā kala i loko o ka ʻenehana DNA recombinant e ʻike i ka hele a me ka hana o ka gene LacZ, kahi e hoʻopili ai i ka β-galactosidase.Kōkua kēia ʻano kānana i ka ʻike ʻana i nā clones i loaʻa ka gene o ka hoihoi.

Nā haʻawina kinetics Enzyme: Pono pū ka ONPG i ke aʻo ʻana i nā kinetics o ka enzyme β-galactosidase.Ma ke ana ʻana i ka wikiwiki o ka hopena enzyme-substrate ma nā ʻano ʻokoʻa substrate, hiki ke hoʻoholo i nā ʻāpana kinetic e like me Michaelis-Menten constants (Km) a me ka nui o ka hopena hopena (Vmax).