4-Nitrophenyl beta-D-galactopyranoside CAS:200422-18-0

Hopena: ʻO ONPG kahi substrate i hoʻohana kūikawā ʻia e ʻike i ke alo a me ka hana o ka enzyme β-galactosidase. Ke kū nei ka enzyme β-galactosidase a hana, hoʻokaʻawale ia iā ONPG i ʻelua huahana: o-nitrophenol a me kahi galactose derivative. ʻO ka hoʻokuʻu ʻia ʻana o ka o-nitrophenol e hopena i kahi loli kala melemele, hiki ke ana ʻia me ka hoʻohana ʻana i ka spectrophotometer.

Noi: He nui nā noi a ONPG i ka biology molekala a me ka noiʻi biochemistry:

Ka hoʻoholo ʻana i ka hana β-galactosidase: Hoʻohana pinepine ʻia ka ONPG e ana a helu i ka hana o ka enzyme β-galactosidase. Hiki ke ana ʻia ka wikiwiki o ka hoʻokumu ʻana o ka o-nitrophenol, ka mea e pili pono ana i ka hana enzyme, ma ke ʻano spectrophotometric.

Ka hōʻike ʻana a me ka hoʻoponopono ʻana o nā gene: Hoʻohana pinepine ʻia ʻo ONPG i nā hoʻokolohua e pili ana i ka hōʻike ʻana o nā gene a me nā haʻawina hoʻoponopono. Hoʻohana pinepine ʻia ia i nā hoʻāʻo protein fusion, e like me ka ʻōnaehana fusion lacZ i hoʻohana pinepine ʻia, e aʻo i ka hōʻike ʻana o nā genes ma lalo o ka mana o nā mea hoʻolaha kikoʻī. Hāʻawi ka hana beta-galactosidase i ana ʻia me ka hoʻohana ʻana iā ONPG i ka ʻike i ka pae o ka hōʻike ʻana o nā gene.

Nānā ʻana no ka hana β-galactosidase: Hiki ke hoʻohana ʻia ʻo ONPG ma ke ʻano he ʻano loiloi colorimetric i ka ʻenehana DNA recombinant e ʻike ai i ke alo a me ka hana o ka gene LacZ, ka mea e hoʻopili ai i ka β-galactosidase. Kōkua kēia ʻano loiloi i ka ʻike ʻana i nā clones i loaʻa ka gene hoihoi.

Nā haʻawina kinetics enzyme: He mea pono nō hoʻi ka ONPG i ke aʻo ʻana i nā kinetics o ka enzyme β-galactosidase. Ma ke ana ʻana i ka wikiwiki o ka hopena enzyme-substrate ma nā ʻano substrate like ʻole, hiki ke hoʻoholo i nā palena kinetic e like me nā Michaelis-Menten constants (Km) a me nā helu hopena kiʻekiʻe loa (Vmax).