5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

ʻO 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) he substrate chromogenic i hoʻohana mau ʻia no ka ʻike ʻana i ka hana beta-glucuronidase (GUS).ʻO GUS kahi enzyme i loaʻa i loko o nā mea ola like ʻole, me ka bacteria, nā mea kanu, a me nā mammals.Hoʻohana pinepine ʻia ʻo X-Gluc i nā hōʻike hoʻolaha GUS a me ka noiʻi biology molecular.

ʻO ka noi nui o X-Gluc i loko o nā ʻenehana staining histochemical, kahi e hiki ai ke ʻike i ka ʻōlelo a me ka hana o ka enzyme GUS.ʻO kēia substrate he cell-permeable a lilo i hydrolyzed e GUS, ka hopena i ka hoʻokumu ʻia ʻana o kahi ʻeke polū a i ʻole huahana hiki ʻole.Hāʻawi kēia ʻulaʻula polū i ka poʻe noiʻi e ʻike a hoʻokaʻawale i ka hana GUS i loko o nā cell, tissues, a me nā mea ola holoʻokoʻa.

Hiki ke hoʻohana ʻia ʻo X-Gluc i nā hoʻokolohua quantitative e ana i ka hana enzyme GUS.Hiki ke hoʻopili ʻia ka ikaika o ke kala uliuli a i ʻole ka nui o ka huahana i hana ʻia me ke kiʻekiʻe o ka hōʻike GUS a i ʻole kāna hana enzymatic.

Hoʻohui ʻia, ua hoʻohana ʻia ʻo X-Gluc i ka noiʻi genetic mea kanu e aʻo i ka hōʻike gene, ka hana hoʻolaha, a me ka hoʻololi ʻana i nā mea kanu.Ua hoʻohana pū ʻia i nā ʻōnaehana bacteria no ka cloning a me ka ʻike ʻana i nā protein fusion GUS.