3-morpholinopropanesulfonic acid hemisodium paʻakai CAS: 117961-20-3

pH buffering: He kūpono ka MOPS-Na i ka mālama ʻana i kahi pH paʻa i ka pae physiological (pH 6.5-7.9). ʻO kona ʻano zwitterionic e ʻae iā ia e kūʻē i nā loli i ka pH ke hoʻohui ʻia nā waikawa a i ʻole nā ​​​​​​bases, e lilo ia i mea pono no ka mālama ʻana i nā kūlana kūpono i nā media moʻomeheu cell a me nā ʻōnaehana hoʻokolohua like ʻole.

Nā haʻawina protein a me nā enzyme: Hoʻohana pinepine ʻia ʻo MOPS-Na i ka hoʻomaʻemaʻe protein, ka wehewehe ʻana, a me ka hoʻopaʻa ʻana. ʻO kona hiki ke hoʻopaʻa a me ka kūlike me nā enzymes a me nā protein he nui e kūpono ai no ka mālama ʻana i ka pH i makemake ʻia i loko o kēia mau kaʻina hana. Hiki ke hoʻohana ʻia ʻo MOPS-Na i nā hoʻāʻo enzyme, kahi e pono ai ka kaohi pH pololei no ke ana pololei ʻana o ka hana enzymatic.

ʻO ka electrophoresis DNA a me RNA: Hoʻohana pinepine ʻia ʻo MOPS-Na ma ke ʻano he buffer i ka electrophoresis gel nucleic acid. Hāʻawi ia i ka pae pH i makemake ʻia a me ka ikaika ionic, e ʻae ana i ka hoʻokaʻawale pono ʻana o nā ʻāpana DNA a me RNA. ʻO ka omo UV haʻahaʻa o MOPS-Na he mea maikaʻi ia i kēia noi, no ka mea, ʻaʻole ia e hoʻopilikia i nā ana spectrophotometric o nā waikawa nucleic.

Pāpaho moʻomeheu kelepona: Hoʻohana ʻia ʻo MOPS-Na i nā pāpaho moʻomeheu kelepona e mālama i ka pH a me ke kaulike osmotic e pono ai no ka ulu ʻana o ke kelepona a me ke ola. ʻO kona kūlike me nā ʻano kelepona like ʻole a me ke ʻano ʻawaʻawa ʻole e lilo ia i mea hoʻopaʻa kūpono no ka mālama ʻana i nā kūlana physiological i nā hoʻokolohua moʻomeheu kelepona.

Noiʻi lāʻau lapaʻau a me ke olaola: Hoʻohana ʻia ʻo MOPS-Na i nā haʻawina lāʻau lapaʻau a me ke olaola like ʻole, e like me ke ana ʻana o ka kinetics enzyme, nā hoʻāʻo pale lāʻau, a me nā haʻawina e pili ana i nā hopena o ka pH ma nā kaʻina hana cellular. Kōkua kona hiki ke buffering i ka hōʻemi ʻana i nā mea hana e pili ana i ka pH a hōʻoia i nā hopena hilinaʻi a hiki ke hana hou ʻia.