HEPES-Na CAS:75277-39-3 Kumukūʻai Mea Hana

Mea hoʻopaʻa: Hoʻohana mau ʻia ka paʻakai paʻakai HEPES ma ke ʻano he mea hoʻopaʻa i nā hoʻokolohua olaola a me ka biochemical.Kōkua ia i ka mālama ʻana i ka pae pH paʻa, ʻoi aku ka nui o ka pae physiological (pH 7.2-7.6).ʻO kona hiki ke hoʻopaʻa i mea waiwai i ka mālama ʻana i nā kūlana kūpono no nā ʻano hopena enzymatic, moʻomeheu cell, a me nā ʻenehana biology molecular.

Moʻomeheu pūnaewele: Hoʻohana nui ʻia ka paʻakai paʻakai HEPES ma ke ʻano he mea hoʻopaʻa i ka media moʻomeheu cell.ʻO ka hiki ke mālama i ka pH paʻa he mea nui ia no ka ulu kūpono a me ka ola o nā cell.ʻOi pinepine ʻia ʻo HEPES ma mua o nā mea hoʻopaʻapaʻa ʻē aʻe no ka mea ʻaʻole ia e hōʻike i nā loli koʻikoʻi i ka pH ke ʻike ʻia i ka atmospheric CO2.

Nā haʻawina Enzyme: He mea pono loa ka paʻakai paʻakai HEPES i nā haʻawina enzymatic kahi e koi ʻia ai ka pH mau a mālama ʻia.Kāohi ia i ka fluctuations drastic i ka pH i ka wā o nā hopena enzymatic, e hōʻoia ana i ka hana enzyme maikaʻi loa.

Electrophoresis: Hoʻohana mau ʻia ka paʻakai paʻakai HEPES ma ke ʻano he mea pale i nā ʻano hana electrophoretic like ʻole me ka polyacrylamide gel electrophoresis (PAGE) a me ka electrophoresis gel agarose.Kōkua ia i ka mālama ʻana i ka pH o ka buffer, ka mea koʻikoʻi no ka hoʻokaʻawale ʻana a me ka nānā ʻana i nā waikawa nucleic a me nā protein.

Nā ho'āʻo biochemical: Hoʻohana pinepine ʻia ka paʻakai paʻakai HEPES i nā ʻano hoʻāʻo biochemical like ʻole, me ka hoʻāʻo ʻana i ka enzyme, ka hoʻohālikelike helu protein, a me nā hōʻike spectrophotometric.Kōkua ia i ka mālama ʻana i ka pae pH i makemake ʻia no nā hopena pololei a hiki ke hana hou ʻia.

Hoʻolāʻau lāʻau: Hoʻohana pū ʻia ka paʻakai paʻakai HEPES i nā ʻano lāʻau lapaʻau ma ke ʻano he mea pale e hoʻopaʻa i nā ʻano lāʻau a mālama i ka pae pH makemake ʻia.