HEPES-Na CAS:75277-39-3 Kumukūʻai Mea Hana

Mea hoʻopili: Hoʻohana pinepine ʻia ka paʻakai sodium HEPES ma ke ʻano he mea hoʻopili i nā hoʻokolohua biological a me biochemical. Kōkua ia i ka mālama ʻana i kahi pae pH paʻa, ʻoi aku hoʻi i ka pae physiological (pH 7.2-7.6). ʻO kona hiki ke hoʻopili e lilo ia i mea waiwai i ka mālama ʻana i nā kūlana kūpono no nā ʻano hana enzymatic like ʻole, ka moʻomeheu cell, a me nā ʻano hana biology molecular.

Moʻomeheu kelepona: Hoʻohana nui ʻia ka paʻakai sodium HEPES ma ke ʻano he mea hoʻopaʻa i nā pāpaho moʻomeheu kelepona. ʻO ka hiki ke mālama i kahi pH paʻa he mea nui ia no ka ulu kūpono a me ke ola o nā kelepona. Makemake pinepine ʻia ʻo HEPES ma mua o nā mea hoʻopaʻa ʻē aʻe no ka mea ʻaʻole ia e hōʻike i nā loli koʻikoʻi i ka pH ke hōʻike ʻia i ka CO2 lewa.

Nā haʻawina enzyme: He mea pono loa ka paʻakai sodium HEPES i nā haʻawina enzymatic kahi e pono ai kahi pH mau a kāohi ʻia. Kāohi ia i nā loli nui o ka pH i ka wā o nā hopena enzymatic, e hōʻoiaʻiʻo ana i ka hana enzyme kūpono.

Electrophoresis: Hoʻohana pinepine ʻia ka paʻakai sodium HEPES ma ke ʻano he mea hoʻopaʻa i nā ʻano hana electrophoretic like ʻole e like me ka polyacrylamide gel electrophoresis (PAGE) a me ka agarose gel electrophoresis. Kōkua ia i ka mālama ʻana i ka pH o ka buffer, he mea nui ia no ka hoʻokaʻawale ʻana a me ka nānā ʻana i nā waikawa nucleic a me nā protein.

Nā hoʻāʻo Biochemical: Hoʻohana pinepine ʻia ka paʻakai sodium HEPES i nā ʻano hoʻāʻo biochemical like ʻole, me nā hoʻāʻo enzyme, nā hoʻāʻo helu protein, a me nā hoʻāʻo spectrophotometric. Kōkua ia i ka mālama ʻana i ka pae pH i makemake ʻia no nā hopena pololei a hiki ke hana hou ʻia.

Ka hoʻohui ʻana i nā lāʻau lapaʻau: Hoʻohana ʻia ka paʻakai sodium HEPES i nā ʻano lāʻau lapaʻau ma ke ʻano he mea hoʻopaʻa i nā ʻano lāʻau lapaʻau a mālama i ka pae pH i makemake ʻia.