NSP-SA-NHS CAS:199293-83-9 Kumukūʻai Mea Hana

ʻO NSP-SA-NHS, ka mea i ʻike ʻia ʻo N-succinimidyl S-acetylthioacetate N-hydroxysuccinimide ester, he pūhui i hoʻohana mau ʻia ma ke ʻano he thiol-specific crosslinking reagent i nā hopena bioconjugation.ʻO kāna hopena nui ka hoʻokumu ʻana i nā paʻa thioester paʻa ma waena o nā pūʻulu thiol i loaʻa ma nā biomolecules, e like me nā protein a i ʻole nā ​​peptides.

ʻO ka noi o NSP-SA-NHS ka mea nui ma ke kahua o ka hoʻololi protein a me ka immobilization.ʻO kekahi o kāna mau noi nui:

Hoʻopaʻa inoa pūmua: Hoʻohana ʻia ka NSP-SA-NHS no ka hoʻopili ʻana i nā lepili, e like me nā dyes fluorescent a i ʻole biotin, i nā protein a i ʻole peptides.ʻAe kēia i ka ʻike, ʻike, a me ka nānā ʻana i nā biomolecules i hōʻailona ʻia i nā ʻano hoʻokolohua biological.

Nā pilina protein-protein: Hiki ke hoʻohana ʻia ka NSP-SA-NHS e hoʻopili i nā protein e pili ana i ke aʻo ʻana i ka pilina protein-protein.Hoʻohana pinepine ʻia kēia i nā ʻenehana e like me ka co-immunoprecipitation a i ʻole ka huki ʻana i lalo e ʻike ai i nā hoa paʻa a i ʻole ke aʻo ʻana i nā paʻakikī protein.

ʻO ka immobilization protein: ʻae ʻo NSP-SA-NHS i ka hoʻopili covalent o nā protein a i ʻole nā ​​peptides ma luna o nā ʻili paʻa, me nā peʻa agarose, nā peʻa magnetic, a i ʻole nā ​​microplates.Pono kēia i nā noi e like me ka hoʻomaʻemaʻe affinity, ka nānā ʻana i ka lāʻau lapaʻau, a i ʻole ka hoʻomohala biosensor.

Hoʻololi ʻili: Hiki ke hoʻohana ʻia ka NSP-SA-NHS e hoʻololi i nā ʻili, e like me nā kiʻi aniani a i ʻole nanoparticles, me nā protein a i ʻole nā ​​peptides, e hana ana i nā papa hana biomolecule-functionalized no nā noi like ʻole e like me nā diagnostics, nā ʻōnaehana lawe lāʻau, a i ʻole nā ​​​​pūnaewele bio-sensing.