4-Methylumbelliferyl-beta-D-glucopyranoside CAS:18997-57-4

ʻO ka hopena o 4-Methylumbelliferyl-beta-D-glucopyranoside (MUG) ʻo ia ke lawelawe ma ke ʻano he substrate no ka enzyme beta-glucosidase. Hoʻopau kēia enzyme i ka pilina glucosidic o MUG, e hopena ana i ka hoʻokuʻu ʻia ʻana o 4-Methylumbelliferone (4-MU). ʻO ka hoʻohana ʻana o MUG ma ke kahua o ka microbiology, ʻoiai no ka ʻike ʻana a me ka ʻike ʻana i nā bacteria e hana ana i ka beta-glucosidase. Hoʻohana pinepine ʻia ʻo MUG i ka ʻike ʻana iā Escherichia coli (E. coli) i loko o ka wai a me nā laʻana meaʻai. Loaʻa iā E. coli ka enzyme beta-glucosidase, hiki ke hydrolyze iā MUG a hana i kahi hōʻailona fluorescent i ke alo o ke kukui ultraviolet (UV). ʻO ka waiwai fluorescent o 4-MU e ʻae i ka maʻalahi o ka ʻike ʻana a me ka helu ʻana. Ke hydrolyzed ʻia ka substrate MUG, hoʻopuka ka 4-MU i hana ʻia i kahi fluorescence polū, e kōkua ana i ka ʻike ʻana i nā bacteria nona ka hana beta-glucosidase. Hoʻohana pinepine ʻia kēia ʻano hana i ka nānā ʻana i ke kaiapuni a me ka hoʻāʻo ʻana i ka palekana o ka meaʻai, no ka mea, hāʻawi ia i kahi ala wikiwiki a koʻikoʻi hoʻi no ka ʻike ʻana i ka haumia bacteria. Ma waho aʻe o kāna noi ʻana i ka microbiology, hiki ke hoʻohana ʻia ʻo MUG e aʻo i ka hana a me ka pale ʻana o ka beta-glucosidase i ka biochemistry a me ka noiʻi biology molecular. Hiki i kona fluorescence ke ana i ka kinetics enzyme a hiki ke hoʻohana ʻia e nānā no nā mea hoʻopaʻa a i ʻole nā ​​​​mea hoʻoulu o ka hana beta-glucosidase. Ma keʻano holoʻokoʻa, he hui versatile ʻo MUG e loaʻa ana ka hoʻohana ākea ma nā kahua o ka microbiology, biochemistry, a me ka biology molecular no ka ʻike ʻana i ka hana beta-glucosidase a me ka ʻike ʻana i nā bacteria e hana ana i kēia enzyme.