4-Nitrophenyl Beta-D-glucuronide CAS:10344-94-2

ʻIke ʻana i ka hana β-glucuronidase: Hoʻohana pinepine ʻia ʻo 4-NPBG ma ke ʻano he substrate chromogenic e loiloi i ke alo a me ka hana o β-glucuronidase i nā ʻano laʻana olaola like ʻole. Hoʻopau ka enzyme i ka pilina glycosidic o 4-NPBG, e hana ana i ka 4-nitrophenol, hiki ke ana ʻia me ka hoʻohana ʻana i ka spectrophotometry.

Nā haʻawina metabolism lāʻau: ʻOiai ʻo β-glucuronidase he kuleana koʻikoʻi i ka metabolism o nā lāʻau a me nā xenobiotics, hiki ke hoʻohana ʻia ʻo 4-NPBG e loiloi i ka hana o kēia enzyme i nā haʻawina metabolism lāʻau. Kōkua ia i ka hoʻomaopopo ʻana i ka nui a me ke kinetics o nā hopena glucuronidation, he mea nui ia no ka hoʻomaʻemaʻe lāʻau a me ka bioavailability.

Nā haʻawina toxicology: Hiki ke hoʻopili ʻia a hoʻokuʻu ʻia kekahi mau hui toxicological ma ke ʻano o nā glucuronide conjugates. Ma ka hoʻohana ʻana i ka 4-NPBG ma ke ʻano he substrate, hiki i nā mea noiʻi ke nānā i ka hana o β-glucuronidase i nā ʻiʻo like ʻole a i ʻole nā ​​​​laina cell e loiloi i ka hiki ke ʻona a i ʻole nā ​​​​hopena maikaʻi ʻole o nā hui.

Nā hōʻailona lapaʻau: Hiki ke hoʻohana ʻia ke ana ʻana o ka hana β-glucuronidase me ka hoʻohana ʻana i ka 4-NPBG i nā hoʻonohonoho lapaʻau e ʻike ai i kekahi mau maʻi a i ʻole nā ​​kūlana. ʻO nā pae maʻamau ʻole a i ʻole ka hana o ka β-glucuronidase e hōʻike paha i kekahi mau maʻi genetic, ka hana pono ʻole o ke akepaʻa, a i ʻole ke kanesa.