P-NITROPHENYL BETA-D-LACTOPYRANOSIDE CAS:4419-94-7

ʻO ka P-Nitrophenyl beta-D-lactopyranoside, ka mea i kapa ʻia ʻo PNPG, he pūhui i hoʻohana pinepine ʻia i nā hoʻokolohua enzymatic e ana i ka hana o ka beta-galactosidase, kahi enzyme e pili ana i ka metabolism carbohydrate.ʻO PNPG kahi substrate synthetic i hiki ke hoʻokaʻawale ʻia e ka beta-galactosidase, ka hopena i ka hoʻokuʻu ʻana i kahi huahana kala melemele.Hiki ke helu ʻia ka nui o ka substrate hydrolysis ma ke ana ʻana i ka hoʻopale ʻana o ka huahana ma kahi lōʻihi hawewe kikoʻī.ʻAe kēia i nā mea noiʻi e loiloi i ka hana a me nā kinetics o ka beta-galactosidase ma nā ʻano ʻano like ʻole, e like me ke aʻo ʻana i ka hana enzyme, ka nānā ʻana i nā mea hoʻopaneʻe enzyme a i ʻole nā mea hoʻoulu, a i ʻole ka loiloi ʻana i nā hopena o nā hoʻololi i ka hana enzyme.

Kauoha a Noi Manao

Huahana Huahana

Hoʻohana a me ka hopena

ʻIke i ka hana beta-galactosidase: Hoʻohana maʻamau ʻia ʻo PNPG i nā hoʻokolohua e ana i ka hana o ka beta-galactosidase, kahi enzyme e hoʻopale i ka hydrolysis o ka lactose i ka glucose a me ka galactose.Hoʻokuʻu ka hydrolysis o PNPG e ka beta-galactosidase i kahi mole p-nitrophenol (pNP), hiki ke ʻike ʻia ma ke ʻano spectrophotometric ma muli o kona kala melemele.

Ka nānā 'ana no nā enzyme inhibitors a me nā mea ho'oikaika: Hiki ke ho'ohana 'ia ka PNPG i ka nānā 'ana i ke ki'eki'e e 'ike i nā pūhui e ho'ololi i ka hana beta-galactosidase.Ma ke ana ʻana i ka nui o ka hydrolysis PNPG i mua o nā pūhui hoʻāʻo like ʻole, hiki i nā mea noiʻi ke ʻike i nā mea hoʻopaneʻe e hoʻemi ana i ka hana enzyme a i ʻole nā mea hoʻoulu e hoʻonui i ka hana enzyme.

Ke aʻo ʻana i nā kinetics enzyme: ʻO ka hydrolysis o PNPG e ka beta-galactosidase ma muli o Michaelis-Menten kinetics, e ʻae ana i nā mea noiʻi e hoʻoholo i nā ʻāpana enzyme koʻikoʻi e like me ka wikiwiki o ka hopena (Vmax) a me ka Michaelis mau (Km).Kōkua kēia ʻike i ka hoʻomaopopo ʻana i ka pili o ka substrate o ka enzyme a me ka hana catalytic.

Nā noiʻi biology molecular: Beta-galactosidase, nāna e hoʻokaʻawale iā PNPG, hoʻohana mau ʻia ma ke ʻano he moʻo nūhou i ka biology molecular.Hoʻohana pinepine ʻia ka substrate PNPG e ʻike a nānā i ka hōʻike ʻana o ka gene reporter, e hāʻawi ana i kahi ala maʻalahi a maʻalahi e loiloi i ka hōʻike ʻana i nā ʻōnaehana hoʻokolohua like ʻole.

Laʻana Huahana

Hoʻopili huahana:

ʻIke hou aku:

Huina	C18H25NO13
Hoʻāʻo	99%
Ka nana aku	Pauda keʻokeʻo
CAS No.	4419-94-7
Hoʻopili	Liʻiliʻi a nui
Ola Pahu	2 makahiki
Waihona	E mālama ma kahi maloʻo a maloʻo
Palapala hōʻoia	ISO.