Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

ʻO Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ka mea maʻamau i hoʻohana ʻia ma ke ʻano he substrate no ka enzyme β-galactosidase.Ke hana ʻo β-galactosidase ma luna o ia mea, ua hydrolyzed ka pūhui, ka hopena i ka hana ʻana o p-nitrophenol a i ʻole o-nitrophenol, nona ka waihoʻoluʻu melemele e hiki ke ʻike maʻalahi.He mea pono kēia ma ke ʻano he substrate no nā hoʻokolohua e ana i ka hana β-galactosidase.

ʻO kahi hoʻohana maʻamau o Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside i loko o nā hōʻike gene reporter.I loko o kēia mau ho'āʻo ʻana, ua hui pū ʻia kekahi gene o ka hoihoi i kahi gene reporter, e like me ka gene beta-galactosidase.A laila nānā ʻia ka hana o ka gene reporter ma ke ana ʻana i ka hana β-galactosidase me ka hoʻohana ʻana iā Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ma ke ʻano he substrate.Hāʻawi kēia i nā mea noiʻi e aʻo i ka hōʻike ʻana a me ka hoʻoponopono ʻana.

Hiki ke hoʻohana ʻia ʻo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside no ka pale ʻana i nā mea hoʻopaneʻe a i ʻole nā ​​​​mea hana o β-galactosidase.Ma ka hoʻāʻo ʻana i nā pūhui like ʻole a i ʻole nā ​​moho lāʻau lapaʻau, hiki i nā mea noiʻi ke ʻike i nā molekala e hoʻololi i ka hana o kēia enzyme, hiki ke loaʻa nā noi therapeutic i kekahi mau maʻi a i ʻole nā ​​kūlana.

Eia kekahi, hiki ke hoʻohana ʻia kēia pūhui i ka hoʻomaʻemaʻe protein.Hoʻohui pinepine ʻia ka β-galactosidase i nā protein hoihoi e hoʻomaʻemaʻe i kā lākou ʻike a hoʻomaʻemaʻe.Hiki ke hoʻohana ʻia ʻo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ma ke ʻano he substrate e nānā a hoʻomaʻemaʻe i kēia mau protein fusion ma ka ʻike ʻana i ka hana β-galactosidase.