ʻO Tris(hydroxymethyl)nitromethane CAS:126-11-4

Hopena:

Ka Mana Hoʻopaʻa: Hana ʻo Tris ma ke ʻano he mea hoʻopaʻa pono ma muli o kona hiki ke ʻae a hāʻawi paha i nā protons, e mālama ana i kahi pae pH paʻa i nā hopena. Hoʻohana pinepine ʻia ia ma ke ʻano he ʻāpana mua i nā ʻōnaehana hoʻopaʻa e hoʻopaʻa i ka pH o nā hāpana olaola a me nā hopena.

Nā noi:

ʻO ke ʻano molekala: Hoʻohana nui ʻia ʻo Tris ma ke ʻano he mea hoʻopaʻa i nā ʻano hana molekala like ʻole, me ka hoʻokaʻawale ʻana o ka DNA a me ka RNA, PCR, gel electrophoresis, a me ka hoʻomaʻemaʻe protein. Kōkua ia i ka mālama ʻana i kahi pH paʻa, e ʻae ana i nā kūlana kūpono no nā hopena enzymatic a me nā pilina molekala.

Moʻomeheu Kelepona: Hoʻohana pinepine ʻia ʻo Tris i nā pāpaho moʻomeheu kelepona e mālama i kahi pH mau a me ke kaulike osmotic, e kākoʻo ana i ka ulu ʻana o nā kelepona olakino a me ke ola.

Kemika Protein: Hoʻohana ʻia ʻo Tris i nā hoʻokolohua kemika protein, e like me ka hoʻoheheʻe ʻana o ka protein, nā hoʻāʻo paʻa protein, a me nā haʻawina hoʻopaʻa protein-ligand. Kōkua ia i ka mālama ʻana i ka pae pH i makemake ʻia, e hōʻoia ana i ka pelu pono ʻana o ka protein a me ka hana.

ʻEnzymology: Hoʻohana ʻia ʻo Tris i nā hoʻāʻo enzymatic like ʻole e hoʻomaikaʻi i nā kūlana pH e pono ai no ka hana enzymatic. Hōʻoiaʻiʻo ia i nā hopena hilinaʻi a hiki ke hana hou ʻia, e hiki ai ke ana pololei i ka kinetics enzyme a me nā haʻawina inhibition.

Nā Hoʻāʻo Biochemical: Hoʻohana ʻia ʻo Tris ma ke ʻano he ʻāpana i nā hoʻāʻo biochemical he nui ma muli o kona mau waiwai buffering. Mālama ia i kahi pH mau i ka wā o nā hoʻāʻo colorimetric, spectrophotometric, a me enzymatic, e hoʻonui ana i ka pololei a me ka hilinaʻi o nā hopena.