ʻO Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside CAS:24404-53-3

Hoʻohana pinepine ʻia ʻo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ma ke ʻano he substrate no ka enzyme β-galactosidase. Ke hana ka β-galactosidase ma luna ona, ua hydrolyzed ka hui, e hopena ana i ka hana ʻana o ka p-nitrophenol a i ʻole o-nitrophenol, nona ke kala melemele i hiki ke ʻike maʻalahi ʻia. ʻO kēia ka mea e pono ai ma ke ʻano he substrate no nā assays e ana i ka hana β-galactosidase.

ʻO kahi hoʻohana maʻamau o Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside i loko o nā hoʻāʻo gene reporter. Ma kēia mau hoʻāʻo, ua hoʻopili ʻia kahi gene hoihoi i kahi gene reporter, e like me ka gene beta-galactosidase. A laila nānā ʻia ka hana o ka gene reporter ma ke ana ʻana i ka hana β-galactosidase me ka hoʻohana ʻana iā Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ma ke ʻano he substrate. ʻAe kēia i nā mea noiʻi e aʻo i ka hōʻike ʻana a me ka hoʻoponopono ʻana o nā gene.

Hiki ke hoʻohana ʻia ʻo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside e nānā no nā mea hoʻopaʻa a i ʻole nā ​​mea hoʻoulu o β-galactosidase. Ma ka hoʻāʻo ʻana i nā hui like ʻole a i ʻole nā ​​​​moho lāʻau lapaʻau, hiki i nā mea noiʻi ke ʻike i nā molekala e hoʻololi i ka hana o kēia enzyme, hiki ke loaʻa nā noi therapeutic i kekahi mau maʻi a i ʻole nā ​​​​kūlana.

Eia kekahi, hiki ke hoʻohana ʻia kēia hui i ka hoʻomaʻemaʻe ʻana i ka protein. Hoʻohui pinepine ʻia ka β-galactosidase i nā protein hoihoi e hoʻomaʻalahi i kā lākou ʻike ʻana a me ka hoʻomaʻemaʻe ʻana. Hiki ke hoʻohana ʻia ʻo Phenyl2,3,4,6-tetra-O-acetyl-1-thio-β-D-galactopyranoside ma ke ʻano he substrate e nānā a hoʻomaʻemaʻe i kēia mau protein fusion ma ka ʻike ʻana i ka hana β-galactosidase.