ʻO 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide he mea hoʻohui i hoʻohana ʻia i loko o nā haʻawina biochemical like ʻole, ʻoi loa no ka ʻike a me ka ʻike ʻana i ka hana enzyme.He substrate ia e hiki ke hoʻoheheʻe ʻia e nā enzyme kikoʻī, ka hopena i ka hoʻokuʻu ʻana i kahi huahana kala a fluorescent paha.
Hoʻohana mau ʻia kēia pūhui i nā hoʻāʻo e ʻike i ke alo a me ka hana o nā enzymes e like me beta-galactosidase a me beta-glucuronidase.Hoʻokaʻawale kēia mau enzymes i nā hui acetyl a me glucosaminide mai ka substrate, e alakaʻi ana i ke kūkulu ʻana i kahi chromophore uliuli a ʻōmaʻomaʻo paha.
ʻO ka hana kūikawā o 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide e hiki ai ke ʻike maʻalahi a me ka helu ʻana o ka hana enzyme.ʻO kona hoʻohana ʻana i nā ʻano hana hoʻokolohua like ʻole, me ka histochemistry, immunohistochemistry, a me nā hoʻāʻo ʻana i ke kelepona, ua kōkua i ka ʻike maikaʻi ʻana i nā hana enzyme.