5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide CAS:4264-82-8

ʻO 5-Bromo-4-chloro-3-indolyl-N-acetyl-beta-D-glucosaminide (X-Gluc) kahi substrate chromogenic i hoʻohana pinepine ʻia no ka ʻike ʻana i ka hana beta-glucuronidase (GUS). ʻO GUS kahi enzyme i loaʻa i nā meaola like ʻole, me nā bacteria, nā mea kanu, a me nā mammals. Hoʻohana pinepine ʻia ʻo X-Gluc i nā hoʻāʻo hōʻike GUS a me ka noiʻi biology molecular.

ʻO ka hoʻohana nui ʻana o X-Gluc i nā ʻano hana hoʻoluʻu histochemical, kahi e hiki ai iā ia ke ʻike i ka hōʻike a me ka hana o ka enzyme GUS. He mea hiki ke hoʻopili ʻia kēia substrate e nā cell a lilo ia i hydrolyzed e GUS, e hopena ana i ka hoʻokumu ʻia ʻana o kahi precipitate polū a i ʻole ka huahana hiki ʻole ke hoʻoheheʻe ʻia. ʻAe kēia hoʻoluʻu polū i nā mea noiʻi e ʻike a hoʻonoho i ka hana GUS i loko o nā cell, nā ʻiʻo, a me nā meaola holoʻokoʻa.

Hiki ke hoʻohana ʻia ʻo X-Gluc i nā hoʻāʻo quantitative e ana i ka hana enzyme GUS. Hiki ke hoʻopili ʻia ka ikaika o ke kala polū a i ʻole ka nui o ka huahana i hana ʻia me ka pae o ka hōʻike ʻana o GUS a i ʻole kāna hana enzymatic.

Eia kekahi, ua hoʻohana ʻia ʻo X-Gluc i ka noiʻi genetic mea kanu e aʻo i ka hōʻike ʻana o ka gene, ka hana hoʻolaha, a me ka hoʻololi ʻana o nā mea kanu. Ua hoʻohana ʻia hoʻi i nā ʻōnaehana bacteria no ka cloning a me ka ʻike ʻana i nā protein fusion GUS.