L-Leucine p-nitroanilide hydrochloride CAS:16010-98-3

Hoʻohana pinepine ʻia ka L-Leucine p-nitroanilide hydrochloride ma ke ʻano he substrate i ka noiʻi biochemical a me nā hoʻāʻo enzyme e ana i ka hana o nā enzymes like ʻole, ʻoiai nā protease a me nā peptidases. Ke ʻoki kēia mau enzymes i ka substrate, hoʻokuʻu ia i ka p-nitroaniline, hiki ke ʻike ʻia ma ke ʻano spectrophotometrically.

ʻO ka wikiwiki o ka loli ʻana o ke kala e pili pono ana i ka hana enzymatic, e ʻae ana i ka helu ʻana o ka hana enzyme i nā laʻana olaola. ʻO kēia ka mea e lilo ai ʻo L-Leucine p-nitroanilide hydrochloride i mea hana pono no ke aʻo ʻana i nā kinetics enzyme, ka nānā ʻana no nā mea hoʻopaʻa enzyme a i ʻole nā ​​​​mea hoʻoulu, a me ka loiloi ʻana i nā hopena o nā kūlana hoʻokolohua a i ʻole nā ​​​​​​hana komo ma ka hana enzyme.

Hiki ke ʻokoʻa ka ʻike o ka L-Leucine p-nitroanilide hydrochloride i nā hoʻāʻo enzymatic like ʻole, e koi ana i ka hoʻonui ʻana no nā ʻōnaehana kikoʻī. Hoʻohana pinepine ʻia ia ma nā ʻano mai 0.5 mM a i 5 mM, ma muli o ka enzyme kikoʻī a me nā kūlana hoʻokolohua.