N-(1-Naphthyl)-3-aminopropanesulfonic Acid Paʻakai Sodium CAS:104484-71-1

Nā Haʻawina Hoʻohālikelike Protein: Loaʻa iā ANS kahi pilina kiʻekiʻe no nā wahi hydrophobic i loko o nā protein. Hoʻohana pinepine ʻia ia e aʻo i ka pelu ʻana o ka protein, ke kūpaʻa, a me nā loli conformational. Hoʻonui ʻia ka fluorescence ANS i ka wā e hoʻopaʻa ai i nā wahi hydrophobic i hōʻike ʻia a i ʻole nā ​​​​protein i pelu hewa ʻia, e lilo ia i mea hana waiwai no ka nānā ʻana i ke ʻano a me ka dynamics o ka protein.

Nā Hana Pilikino-Ligand: Hiki ke hoʻohana ʻia ʻo ANS e noiʻi i nā hana pilikino-ligand, e like me ke kāpili ʻana o nā mole liʻiliʻi a i ʻole nā ​​lāʻau lapaʻau i nā protein. ʻO ka hoʻonui ʻia a i ʻole ka emi ʻana o ka fluorescence ANS i ke alo o kahi ligand hiki ke hāʻawi i ka ʻike e pili ana i ka pilina pili a me ka wahi o ka wahi pili.

Nā Haʻawina Membrane: Ma muli o kona ʻano hydrophobic, hoʻohana pinepine ʻia ʻo ANS e aʻo i nā protein membrane a me nā pilina lipid-protein. Hiki iā ia ke nānā i nā waiwai lipid bilayer, hoʻoholo i ka topology protein membrane, a nānā i nā loli i ka fluidity membrane.

Loiloi o ka Denaturation Protein: Hiki ke hoʻohana ʻia ka fluorescence ANS e loiloi i ka denaturation protein a i ʻole ka wehe ʻana, e like me nā kaʻina hana denaturation i hoʻoulu ʻia e ka wela a i ʻole kemika. ʻIke pinepine ʻia ka piʻi ʻana o ka fluorescence ANS i ka wā e hōʻike ʻia ai nā wahi hydrophobic.

Ka ʻIke ʻana i ka Hōʻuluʻulu Protein: Ua ʻike ʻia ʻo ANS e hoʻopaʻa i nā hōʻuluʻulu protein, e like me nā fibrils amyloid a i ʻole nā ​​​​kino hoʻokomo. Hiki ke hoʻohana ʻia ka hoʻonui ʻana o ka ikaika fluorescence ANS i ke alo o nā hōʻuluʻulu protein e nānā i ka hoʻokumu ʻia ʻana a me ka holomua o kēia mau ʻano.

Nā Haʻawina Hoʻopaʻa me nā ʻAkika Nucleic: Hiki i ka ANS ke launa pū me nā ʻakika nucleic, ʻoiai ka DNA kaula pālua, a hōʻike i ka fluorescence. Ua hoʻohana ʻia e aʻo i nā pilina DNA-protein a me nā loli conformational DNA.

Ka Lepili ʻana i ka Fluorescent: Hiki ke hoʻohui ʻia ʻo ANS a i ʻole e hoʻopili ʻia me ke ʻano covalently i nā molekala hoihoi e hana i nā probes fluorescent a me nā lepili. Hiki i kēia ke ʻike maka ʻia a me ka hahai ʻana i nā molekala kikoʻī a i ʻole nā ​​​​​​hale cellular.