ʻAkika 3-(cyclohexylamino)-2-hydroxy-1-propanesuhicic CAS:73463-39-5

ʻO CAPSO (3-(cyclohexylamino)-2-hydroxypropanesulfonic acid) kahi buffer zwitterionic i hoʻohana pinepine ʻia i ka biochemistry a me ka biology molecular. Ua kaulana ia no kona hiki ke hoʻopaʻa kiʻekiʻe ma luna o kahi laulā pH ākea a ua manaʻo ʻia he pani maikaʻi no nā buffers ʻē aʻe e like me MOPS a me MES.

ʻO ka hopena nui o CAPSO ʻo ia kona hiki ke mālama i kahi pH paʻa i nā hoʻokolohua olaola. Hana ia ma ka hāʻawi ʻana a i ʻole ka ʻae ʻana i nā protons e hōʻemi i nā loli i ka pH i hoʻokumu ʻia e nā waikawa a i ʻole nā ​​​​​​base i hoʻohui ʻia. ʻO kona waiwai pKa ma kahi o 9.8, ka mea e lilo ai ia i mea pale kūpono no nā hoʻokolohua ma ka pae pH o 8.2-9.6.

Hoʻohana pinepine ʻia ʻo CAPSO i nā noi e like me ka hoʻomaʻemaʻe protein, nā hoʻāʻo enzyme, a me ka electrophoresis. ʻO kona kūpaʻa a me ka liʻiliʻi o ka hoʻopilikia ʻana i nā hopena olaola e lilo ia i mea pono no ka mālama ʻana i nā kūlana kūpono no nā hopena biochemical. Eia kekahi, hoʻohana pinepine ʻia ʻo CAPSO no ke aʻo ʻana i ke ʻano o ka protein kumu, ka pelu ʻana o ka protein, a me ke kūpaʻa.