3-HYDROXY-4-(5-NITROPYRIDYLAZO)PROPYLANI CAS:143205-66-7

He ʻano probe fluorescent ia e loaʻa ana kahi hui nitrobenzoxadiazole (NBD), kahi e hōʻike ana i nā waiwai fluorescence ikaika. ʻAe ka hui aldehyde i ka lepili kikoʻī a me ka ʻike ʻana i nā biomolecules e like me nā protein, nā nucleic acid, a me nā lipids.

Hoʻohana pinepine ʻia ʻo NBD-aldehyde ma ke ʻano he dye reactive i nā assays no ka ʻike ʻana a me ka helu ʻana i nā hopena conjugation protein a peptide paha, nā hoʻololi protein, a me nā pilina protein-protein. Hiki iā ia ke lepili i nā hui amino manuahi ma nā protein a i ʻole peptides, e hana ana i nā pilina covalent paʻa. ʻO kēia ka mea e pono ai no nā noi e like me immunocytochemistry, immunohistochemistry, a me ke komohana blotting.

Hiki ke hoʻonāukiuki ʻia ka fluorescence o NBD-aldehyde me ka mālamalama ultraviolet a polū paha, a hoʻopuka ia i ka fluorescence ʻōmaʻomaʻo. Hiki i kēia waiwai ke ʻike maka ʻia a me ka helu ʻana o nā biomolecules i hōʻailona ʻia me ka hoʻohana ʻana i ka microscopy fluorescence a i ʻole nā ​​​​ʻano hana spectroscopy.