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3-NITROPHENYL-BETA-D-GALACTOPYRANOSIDE CAS:3150-25-2

ʻO 3-Nitrophenyl-beta-D-galactopyranoside (ONPG) kahi substrate i hoʻohana mau ʻia i nā hoʻokolohua enzymatic e ʻike a ana i ka hana o ka beta-galactosidase.Ke loaʻa ka beta-galactosidase a ikaika, hydrolyzes ia i ka ONPG, e hoʻokuʻu ana i kahi hua melemele i kapa ʻia ʻo 3-nitrophenol.Hiki ke ana ʻia ka ikaika o ka waihoʻoluʻu melemele i hana ʻia me ka spectrophotometrically, e ʻae ana i ka nui o ka hana beta-galactosidase.Hoʻohana pinepine ʻia ʻo ONPG i ka molecular biology a me ka noiʻi microbiology, a me nā diagnostics clinical, e aʻo ai i ka hōʻike gene, ka hana protein, ka ʻike bacterial, a me ka viability cell.

 


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Hoʻohana a me ka hopena

ʻIke i ka hana beta-galactosidase: Hoʻohana pinepine ʻia ʻo ONPG no ka hoʻoholo ʻana i ka hele ʻana a me ka hana o ka beta-galactosidase i loko o nā laʻana olaola like ʻole, e like me nā moʻomeheu bacteria a i ʻole cell lysates.Hiki ke ana maʻalahi ka hana ʻana o ka o-nitrophenol, nona ka waihoʻoluʻu melemele me ka spectrophotometer.

Nā haʻawina hōʻike Gene: Hoʻohana mau ʻia ʻo ONPG i ka noiʻi molecular biology e aʻo i ka ʻōlelo gene.Ma ka hoʻohui ʻana i ka mea hoʻolaha o kahi moʻo hoihoi me ka gene encoding beta-galactosidase, hiki i nā mea noiʻi ke ana i ka hana o kēia mea hoʻolaha ma o ka hoʻohui ʻana i ka ONPG a me ka helu ʻana i ka hana o-nitrophenol.ʻO kēia ala, i ʻike ʻia he beta-galactosidase reporter assay, hāʻawi i ka ʻike e pili ana i ka hana transcriptional o kahi gene.

ʻIke ʻana i ka maʻi bacteria: Hoʻopuka kekahi mau huakini i ka beta-galactosidase, ʻaʻole hana kekahi.Hiki ke hoʻohana ʻia ka ONPG me nā hoʻokolohua biochemical ʻē aʻe e ʻike ai i nā ʻano bacteria ma muli o ko lākou hiki ke hydrolyze i ka ONPG.Hoʻohana pinepine ʻia kēia ʻano hana i nā diagnostics clinical a me nā keʻena microbiology.

Ke nānā ʻana no nā mea hoʻopaneʻe enzyme a i ʻole nā ​​mea hoʻoulu: Hiki ke hoʻohana ʻia ka ONPG e nānā i nā pūhui e hoʻololi i ka hana o ka beta-galactosidase.Ma ke ana ʻana i ka hana enzyme i mua o nā pūhui like ʻole, hiki i nā mea noiʻi ke ʻike i nā mea hoʻopaneʻe a i ʻole nā ​​​​mea hoʻoulu e hiki ke noiʻi hou ʻia no ko lākou hiki ke hoʻōla.

Hoʻopili huahana:

6892-68-8-3

ʻIke hou aku:

Huina C12H15NO8
Hoʻāʻo 99%
Ka nana aku Keʻokeʻopauda
CAS No. 3150-25-2
Hoʻopili Liʻiliʻi a nui
Ola Pahu 2 makahiki
Waihona E mālama ma kahi maloʻo a maloʻo
Palapala hōʻoia ISO.

 


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