4-Nitrophenyl-beta-D-glucopyranoside CAS:2492-87-7

Ka hoʻāʻo ʻana i ka hana enzyme: Hiki ke hoʻohana ʻia ka hydrolysis o 4-Nitrophenyl-beta-D-glucopyranoside e nā enzymes e like me β-glucosidase e ana i ka hana enzyme. Hiki ke helu ʻia ka hoʻokuʻu ʻia ʻana o 4-nitrophenol me ka hoʻohana ʻana i ka spectrophotometry, e ʻae ana i ka hoʻoholo ʻana i nā kinetics enzyme a me nā haʻawina inhibition.

Nā hoʻāʻo hōʻike gene a me nā mea hōʻike: Hiki ke hoʻopili ʻia ka 4-Nitrophenyl-beta-D-glucopyranoside me nā lepili molekala kikoʻī i hoʻokomo ʻia i loko o nā protein fusion. A laila hiki ke helu maʻalahi ʻia ka hana enzyme o ka protein fusion ma ke ana ʻana i ka hoʻokuʻu ʻia ʻana o 4-nitrophenol, e hāʻawi ana i ka ʻike i ka hōʻike ʻana o ka gene a me nā pilina protein-protein.

ʻIke ʻana i ka lāʻau lapaʻau a me ka nānā ʻana i ka throughput kiʻekiʻe: Hiki ke hoʻohana ʻia ka hydrolysis o 4-Nitrophenyl-beta-D-glucopyranoside ma ke ʻano he hoʻāʻo hana pani no ka ʻike ʻana i ka lāʻau lapaʻau. ʻAe ia i ka nānā wikiwiki ʻana o nā hale waihona puke nui e ʻike i nā mea hoʻopaʻa enzyme a i ʻole nā ​​​​mea hoʻoulu.

Hoʻāʻo ʻana i ka maʻi: Pili kekahi mau maʻi me nā loli i kekahi mau hana enzyme. Ma ka hoʻohana ʻana i ka hydrolysis o 4-Nitrophenyl-beta-D-glucopyranoside e nā enzymes kikoʻī ma ke ʻano he hoʻāʻo diagnostic, hiki ke ʻike ʻia a nānā ʻia paha kēia mau maʻi. Hoʻokomo pū kēia i nā kūlana e pili ana i nā maʻi mālama lysosomal a me nā maʻi hepatobiliary.