4-NITROPHENYL-ALPHA-D-MANNOPYRANOSIDE CAS:10357-27-4

Nā substrates enzyme: Hiki ke hoʻohana ʻia ʻo 4NPM ma ke ʻano he substrate no nā enzymes like ʻole, me nā glycosidases a me nā enzymes pili. Hoʻokaʻawale kēia mau enzymes i ka pilina glycosidic ma waena o ka mannose a me 4NPM, e hopena ana i ka hoʻokuʻu ʻia ʻana o ka nitrophenyl moiety. Hiki ke ana ʻia ka nui o ka substrate hydrolysis spectrophotometrically ma ka nānā ʻana i ka absorbance o ka hui nitrophenyl i hoʻokuʻu ʻia ma kahi nalu kikoʻī. Hiki i kēia i nā mea noiʻi ke loiloi i ka hana enzyme a me nā kinetics.

Nā hoʻāʻo no ka metabolism carbohydrate: Ma ka hoʻohana ʻana i ka 4NPM ma ke ʻano he substrate, hiki i nā mea noiʻi ke aʻo i ka hana o nā enzymes e pili ana i ka metabolism carbohydrate, e like me alpha-mannosidases. Hoʻohui kēia mau enzymes i nā pilina glycosidic i loko o nā hui e loaʻa ana ka mannose, a hiki ke ana ʻia kā lākou hana ma ka nānā ʻana i ka hoʻokuʻu ʻia ʻana o ka nitrophenyl moiety mai 4NPM.

Nā haʻawina Glycosylation: Hiki ke hoʻohana ʻia ʻo 4NPM i nā assays e noiʻi i nā enzymes e pili ana i nā kaʻina hana glycosylation. ʻO Glycosylation ke kaʻina hana o ka hoʻopili ʻana i nā molekala kō i nā protein a i ʻole nā ​​molekala ʻē aʻe, a he nui nā enzymes e pili ana i kēia kaʻina hana. Ma ka hoʻohana ʻana iā 4NPM ma ke ʻano he substrate acceptor, hiki i nā mea noiʻi ke ana i ka hoʻoili ʻana o kahi ʻāpana kō i 4NPM e nā enzymes kikoʻī e pili ana i nā hopena glycosylation.

Nānā ʻana no nā mea hoʻopaʻa enzyme a i ʻole nā ​​mea hoʻoulu: Hiki ke hoʻohana ʻia ʻo 4NPM i nā hoʻāʻo loiloi throughput kiʻekiʻe e ʻike i nā hui e kāohi a hoʻoulu paha i nā enzyme kikoʻī. Ma ka loiloi ʻana i ka hopena o nā hui hoʻāʻo ma ka hydrolysis a i ʻole ka hoʻololi ʻana o 4NPM e nā enzymes i manaʻo ʻia, hiki i nā mea noiʻi ke ʻike i nā mea lapaʻau kūpono a i ʻole nā ​​​​​​probes kemika pono no ke aʻo ʻana i ka hana enzyme.