3,3,5,5-Tetramethylbenzidine dihydrochloride hydrate CAS:207738-08-7

ʻO 3,3',5,5'-Tetramethylbenzidine dihydrochloride (TMB) kahi substrate i hoʻohana nui ʻia i nā enzyme-linked immunosorbent assays (ELISAs) a me nā biochemical assays ʻē aʻe. Ke hoʻopau ʻia ʻo TMB e ka enzyme horseradish peroxidase (HRP) i ke alo o ka hydrogen peroxide, hana ia i kahi kala polū. Hoʻohana ʻia kēia hoʻololi kala e ʻike i ke alo o nā antibodies i hōʻailona ʻia e HRP a i ʻole nā ​​​​protein ʻē aʻe, e hōʻike pinepine ana i ke alo o kahi antigen kikoʻī i loko o kahi laʻana. ʻO ke kala polū i hana ʻia e ka hopena e helu pinepine ʻia me ka hoʻohana ʻana i kahi spectrophotometer, kahi e hiki ai ke ana i ka ikaika o ka hoʻololi kala a hāʻawi i kahi ala e hoʻopili ai i ka ikaika o ke kala me ka nui o ka molekala target i loaʻa i loko o ka laʻana. He nui nā noi o TMB i nā assays diagnostic, noiʻi, a me ka hoʻomohala lāʻau lapaʻau, ʻoi aku hoʻi i ka ʻike ʻana i nā antibodies, hormones, cytokines, a me nā biomolecules ʻē aʻe. ʻO kona ʻike, kūpaʻa, a me ka maʻalahi o ka hoʻohana ʻana e lilo ia i mea hana waiwai nui i nā hoʻonohonoho hoʻokolohua like ʻole no ka ʻike ʻana a me ka helu ʻana o nā molekala kikoʻī.