3,3′-Diaminobenzidine CAS:91-95-2

ʻO ka hopena o 3,3'-Diaminobenzidine (DAB) ka hana ʻana i kahi kala ʻeleʻele ke pane ia me nā enzymes peroxidase. ʻAe kēia hoʻololi kala i ka nānā ʻana i nā antigens target a i ʻole biomolecules hoihoi i ka immunohistochemistry (IHC) a me nā hoʻāʻo staining immunocytochemistry (ICC). Hoʻohana pinepine ʻia ʻo DAB i ka noiʻi biomedical a me nā noi diagnostic e ʻike a hoʻokomo i nā antigens a i ʻole nā ​​​​protein kikoʻī i loko o nā laʻana biological. Ma ka hoʻohui ʻana iā DAB ma ke ʻano he substrate a me ka hopena ma hope me nā enzymes peroxidase, ua hoʻokumu ʻia kahi precipitate brown ma kahi o nā antigens target. He mea pono loa ka hoʻopili ʻana o ka staining DAB i ke aʻo ʻana i nā maʻi a me nā kūlana like ʻole. Hiki iā ia ke hiki i nā mea noiʻi a me nā pathologists ke nānā a kālailai i ka hoʻolaha ʻana, ka nui, a me ka localization o nā biomolecules kikoʻī i loko o nā ʻiʻo a i ʻole nā ​​​​​​cell. Kōkua kēia ʻike i ka hoʻomaopopo ʻana i nā ʻano hana maʻi, ke aʻo ʻana i nā kaʻina hana cellular, a me ka ʻike ʻana i nā pahuhopu diagnostic a therapeutic paha. Eia kekahi, hiki ke hoʻohana ʻia ka DAB staining i nā ʻano hana molekala like ʻole e noiʻi i nā pilina protein-protein, ʻike i nā ʻano hōʻike protein, hoʻoholo i ke alo o nā māka kikoʻī, a loiloi i ka pono o nā hana therapeutic. Ma muli o kona staining hilinaʻi a paʻa, a me kona kūlike me nā ʻōnaehana ʻike like ʻole a me nā ʻano hana microscopy, ua lilo ʻo DAB i substrate chromogenic i hoʻohana nui ʻia i ka immunohistochemistry a me nā immunocytochemistry assays.