3,3′,5,5′-Tetramethylbenzidine sulfate CAS:54827-18-8

ELISA: Hoʻohana nui ʻia ia ma ke ʻano he substrate no ka horseradish peroxidase (HRP) i nā hoʻāʻo ELISA. I ke alo o kahi molekala i manaʻo ʻia, e like me ka antigen a i ʻole antibody, hoʻoulu ka enzyme HRP i ka oxidation o 3,3',5,5'-Tetramethylbenzidine sulfate, e alakaʻi ana i ka hoʻokumu ʻia ʻana o kahi huahana polū. ʻO ka ikaika o ke kala e pili pono ana i ka nui o ka molekala i manaʻo ʻia, e ʻae ana i ka nānā ʻana i ka quantitative.

Nā hoʻāʻo hana enzyme: Hiki ke hoʻohana ʻia ka 3,3',5,5'-Tetramethylbenzidine sulfate e ana i ka hana enzyme. Ma ka hoʻomoʻa ʻana i kahi hāpana enzyme me ka substrate, hiki ke nānā ʻia ka hopena enzymatic e ka loli o ke kala. ʻO ka hana enzyme kiʻekiʻe e alakaʻi i kahi kala polū ʻoi aku ka ikaika, e hiki ai ke hoʻoholo i nā kinetics enzyme a me nā haʻawina inhibition.

Immunohistochemistry: Hiki ke hoʻohana ʻia no nā kaʻina hana hoʻoluʻu i ka immunohistochemistry (IHC). Hoʻohana ʻia ia ma ke ʻano he chromogen no nā antibodies mua a lua paha i hōʻailona ʻia e ka horseradish peroxidase, e hopena ana i ka hoʻokumu ʻia ʻana o kahi waihona polū i ʻike ʻia ma kahi o ka pilina antigen-antibody.

Noiʻi Biochemical: Loaʻa nā noi o 3,3',5,5'-Tetramethylbenzidine sulfate i nā haʻawina noiʻi biochemical like ʻole, ʻoi aku hoʻi i ka ʻike ʻana a me ke ana ʻana o nā hana enzyme, nā pilina protein-protein, a me nā pilina protein-DNA.