4-Benzylamino-7-nitrobenz-2-oxa-1,3-diazole CAS:18378-20-6

ʻŌmole Fluorescent: Hoʻohana nui ʻia ʻo BANA ma ke ʻano he ʻōmole fluorescent i nā noiʻi olaola a me nā noi kiʻi. Hōʻike ia i ka hoʻokuʻu ʻana o ka fluorescence ma ka ʻāpana ʻulaʻula o ka spectrum ma hope o ka hoʻonāukiuki ʻia me ka ultraviolet a i ʻole ke kukui ʻike ʻia. ʻO kēia waiwai e lilo ia i mea pono no ka lepili ʻana a me ka nānā ʻana i nā molekala kikoʻī a i ʻole nā ​​​​​​hale i loko o nā ʻōnaehana olaola.

Ka Nānā ʻAkika Amino: Ua hoʻohana ʻia ʻo BANA ma ke ʻano he reagent i nā ʻano loiloi waikawa amino. Hoʻopili ia me ka phenylalanine a me ka tyrosine e hana i nā derivatives fluorescent e hiki ke ana ʻia a helu ʻia. Kōkua kēia ʻano hana loiloi i ka ʻike ʻana a me ka helu ʻana o nā waikawa amino i nā laʻana like ʻole, e like me nā hydrolysates protein.

ʻIke ʻana i ka Enzyme: Ua hoʻohana ʻia ʻo BANA ma ke ʻano he substrate no ka ʻike ʻana i kekahi mau enzymes, ʻoiai kahi enzyme bacteria i kapa ʻia ʻo trypsin-like serine protease. Hoʻopau ka enzyme i ka mole BANA, e alakaʻi ana i ka hoʻokuʻu ʻia ʻana o kahi huahana fluorescent e hiki ke ana ʻia. Ua hoʻohana ʻia kēia ʻano hana i ka ʻike ʻana i nā maʻi periodontal, ma ke ʻano he māka no ka hana o kekahi mau bacteria e pili ana i kēia mau maʻi.

Biomarker i ke Ola Waha: Ua aʻo ʻia hoʻi ʻo BANA ma ke ʻano he biomarker kūpono no ka loiloi olakino waha. ʻO kekahi mau bacteria, e like me Porphyromonas gingivalis, e hana i kahi enzyme i kapa ʻia ʻo trypsin-like activity (TLA), ka mea e hydrolyzes i ka BANA. Hiki i ke alo a me ka pae o TLA ke hōʻike i ke alo a me ke koʻikoʻi o nā maʻi periodontal, e kōkua ana i kā lākou ʻike a me ka nānā ʻana.