5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt CAS:129541-41-9 Kiʻi i hōʻike ʻia

5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide paʻakai sodium CAS:129541-41-9

ʻO ka paʻakai sodium 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide kahi hui kemika i hoʻohana pinepine ʻia i ka noiʻi hale hana a me nā diagnostics. Ua kapa pinepine ʻia ʻo X-Gluc a hoʻohana nui ʻia ma ke ʻano he substrate no ka ʻike ʻana i ka hana enzyme beta-glucuronidase.

Ke loaʻa ka beta-glucuronidase, ʻoki ia i ka pilina glucuronide ma X-Gluc, e hopena ana i ka hoʻokuʻu ʻia ʻana o kahi waihoʻoluʻu polū i kapa ʻia ʻo 5-bromo-4-chloro-3-indolyl. Hoʻohana pinepine ʻia kēia hopena e ʻike maka a spectrophotometric paha i ka hōʻike ʻana o ka enzyme beta-glucuronidase i loko o nā cell a i ʻole nā ʻiʻo.

Hoʻomaikaʻi ke ʻano paʻakai sodium o X-Gluc i kona solubility i loko o nā hopena wai, e hoʻomaʻalahi ana i kona hoʻohana ʻana i nā hoʻāʻo hoʻokolohua. Hoʻohana nui ʻia ʻo X-Gluc i ka noiʻi biology molecular e aʻo i ka hōʻike ʻana o ka gene, ka hana hoʻolaha, a me nā hoʻāʻo gene reporter. Hiki ke hoʻohana ʻia e ʻike i ke alo o nā meaola e hana ana i ka beta-glucuronidase, e like me kekahi mau bacteria, i nā haʻawina microbiological.

Kauoha a Noi paha i ka ʻŌlelo Kūʻai

Nā kikoʻī huahana

Nā Lepili Huahana

Hoʻohana a me ka hopena

ʻIke ʻana iā GUS: Ua ʻoki ʻia ʻo X-Gluc e ka enzyme GUS i loko o kahi hui polū hiki ʻole ke hoʻoheheʻe ʻia i ʻike ʻia ʻo 5-bromo-4-chloro-3-indole (X-Ind). ʻAe kēia hopena i ka nānā ʻana a me ka helu ʻana o ka hana GUS i loko o nā pūnaewele a me nā ʻiʻo.

Nā haʻawina hōʻike gene: Hoʻohana ʻia ʻo X-Gluc ma ke ʻano he molekala hōʻike i nā haʻawina hōʻike gene. Ma ka hoʻohui ʻana i ka gene GUS i kahi mea hoʻolaha hoihoi, hiki i nā mea noiʻi ke hoʻoholo i ka hana a me ke ʻano hōʻike spatial-temporal o ka mea hoʻolaha ma o ka ʻike ʻana i ka hana GUS me ka hoʻohana ʻana iā X-Gluc.

Ka loiloi ʻana i nā mea kanu transgenic: Hoʻohana nui ʻia ka ʻōnaehana gene reporter GUS i ka biology molecular mea kanu. ʻAe ka hoʻoluʻu X-Gluc i nā mea noiʻi e ʻike a aʻo i nā ʻano hōʻike transgene i nā mea kanu. Kōkua kēia i ka hoʻomaopopo ʻana i ka hoʻoponopono ʻana o nā gene, ka hōʻike ʻana o nā ʻiʻo, a me ka biology ulu i nā mea kanu.

ʻEnekinia hoʻoilina: Hoʻohana ʻia ʻo X-Gluc ma ke ʻano he māka hiki ke koho ʻia i nā hoʻokolohua ʻenekinia hoʻoilina. Ma ka hoʻopili ʻana i ka gene GUS i kahi gene hoihoi ʻē aʻe, hiki ke hoʻohana ʻia ka hoʻoluʻu ʻana o X-Gluc e ʻike i ka hoʻololi kūleʻa a me ka hoʻohui ʻana o nā genes i makemake ʻia i loko o ka meaola.

Noiʻi microbiology: Hiki ke hoʻohana ʻia ʻo X-Gluc e ʻike a ʻike i nā bacteria hana GUS. Loaʻa ka enzyme GUS i nā ʻano bacteria like ʻole, a ʻo ka hoʻoluʻu ʻana me X-Gluc e ʻae ai i ka nānā ʻana a me ka ʻike ʻana i nā bacteria GUS-positive i nā haʻawina microbiological.

Laʻana Huahana

Hoʻopili Huahana:

ʻIke Hou Aʻe:

Hoʻonohonoho ʻana	C14H14BrClNNaO7
Hoʻāʻo	99%
helehelena	Pauka keʻokeʻo
Helu CAS.	129541-41-9
Hoʻopili ʻana	Liʻiliʻi a nui
Ola Papa	2 mau makahiki
Ka Waihona	E mālama i kahi maloʻo a me kahi anuanu
Palapala hōʻoia	ISO.