8-Anilino-1-naphthalenesulfonic acid ammonium paʻakai CAS: 28836-03-5

anakahi pH: Ke loli nei ka hui i kona piko hoʻokuʻu fluorescence ma muli o ka pH o ka hopena i loko. ʻO kēia waiwai e hoʻolilo iā ia i mea pono ma ke ʻano he anakahi pH i nā noi like ʻole, e like me ka nānā ʻana i nā loli pH i nā laʻana olaola a i ʻole ke aʻo ʻana i nā hopena enzymatic.

Ka loiloi ʻana i ke ʻano o ka protein: Hiki i ka paʻakai ammonium 8-Anilino-1-naphthalenesulfonic acid ke hoʻopaʻa i nā protein a loli i ka ikaika fluorescence a i ʻole nā ​​​​​​waiwai spectral ma muli o ke ʻano o ka protein. ʻO kēia ka mea e lilo ai ia i mea hana pono no ke aʻo ʻana i ka pelu ʻana o ka protein, denaturation, a me nā loli conformational.

Nā pilina protein-ligand: Hiki ke hoʻohana ʻia ka hui e aʻo i nā pilina protein-ligand. Ma ka nakinaki ʻana i kahi protein a me ka loli ʻana o ka fluorescence ma luna o ka nakinaki ʻana o ka ligand, hiki i nā mea noiʻi ke noiʻi i ka pilina pili, kinetics, a me ke ʻano hana o nā ligands like ʻole.

ʻIke ʻana i ka ion: Ua hoʻohana ʻia ka paʻakai ammonium 8-Anilino-1-naphthalenesulfonic acid e ʻike a helu i nā ion metala like ʻole, e like me ka calcium, magnesium, a me ka zinc. Hiki ke hoʻololi ʻia nā waiwai fluorescence o ka hui ma o ke alo o kēia mau ion, e hāʻawi ana i kahi ʻano koʻikoʻi a koho no kā lākou ʻike ʻana.

Ka nānā ʻana i ke kaiapuni: Ua hoʻohana ʻia ka hui no nā kumu nānā ʻana i ke kaiapuni, e like me ka ʻike ʻana i nā mea haumia a me ka nānā ʻana i nā loli i ka maikaʻi o ka wai. ʻO kona mau waiwai fluorescence e hiki ai ke ʻike wikiwiki a koʻikoʻi i nā mea haumia i loko o nā hāpana wai.

Kemika loiloi: Hoʻohana ʻia ka paʻakai ammonium 8-Anilino-1-naphthalenesulfonic acid i nā ʻano hana loiloi like ʻole, e like me ka fluorescence spectroscopy a me ka chromatography wai hana kiʻekiʻe (HPLC), no ka helu ʻana a me ke ʻano o nā hui i nā hui paʻakikī.