AMPPD CAS:122341-56-4 Kumukūʻai Mea Hana

AMPPD, pōkole no ʻO 3-(2′-spiroadamantane)-4-methoxy-4-(3″-phosphoryloxy)phenyl-1,2-dioxetane, he substrate chemiluminescent i hoʻohana pinepine ʻia i nā noi biological a me immunoassay. Hoʻohana nui ʻia ia i ka noiʻi biology molecular, diagnostics, a me nā ʻepekema ola.

Ua hoʻolālā kūikawā ʻia ʻo AMPPD e hoʻohana pū ʻia me nā alkaline phosphatase enzyme conjugates. Ke hōʻike ʻia i ka alkaline phosphatase, hana ʻo AMPPD i kahi hopena enzymatic cleavage wikiwiki a maikaʻi hoʻi, e alakaʻi ana i ka hoʻokuʻu ʻana o ka mālamalama.

ʻIke ʻia ke kukui i hoʻopuka ʻia mai AMPPD me ka hoʻohana ʻana i nā ʻōnaehana kiʻi koʻikoʻi a i ʻole nā ​​luminometers, e ʻae ana i ka helu ʻana a me ka nānā ʻana i nā molekala i manaʻo ʻia a i ʻole nā ​​​​analytes. Hāʻawi kēia ʻano ʻike e pili ana i ka chemiluminescence i ka ʻike kiʻekiʻe, ka walaʻau hope haʻahaʻa, a me kahi laulā dynamic ākea.

Ma muli o kona kūpaʻa a me ka hōʻailona lōʻihi, kūpono loa ʻo AMPPD no nā noi e like me Western blotting, ELISA (enzyme-linked immunosorbent assay), DNA hybridization, a me ka in situ hybridization. Hiki iā ia ke ʻike a kālailai i nā protein, nā nucleic acid, nā antibodies, a me nā biomolecules ʻē aʻe.

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