ʻO Avidin CAS:1405-69-2 Kumukūʻai Mea Hana

Hoʻomaʻemaʻe Pūmua: Hiki ke hoʻopaʻa ʻia ʻo Avidin ma luna o kahi kākoʻo paʻa, e like me ka resin chromatography a i ʻole nā ​​​​mīkini magnetic, a hoʻohana ʻia e hopu i nā protein biotinylated a i ʻole nā ​​​​​​waikawa nucleic. Ua kapa ʻia kēia ʻano hana ʻo avidin affinity chromatography, a hiki iā ia ke hoʻomaʻemaʻe i nā molekala pahuhopu kikoʻī ma ke ʻano kikoʻī loa.

Nā Probes Biotinylated: Hoʻohui pinepine ʻia ʻo Avidin me nā enzymes, fluorophores, a i ʻole nā ​​​​lepili ʻē aʻe a hoʻohana ʻia ma ke ʻano he reagent ʻike i nā hoʻāʻo like ʻole. Hiki ke ʻike ʻia nā probes Biotinylated, e like me nā antibodies a i ʻole nā ​​​​probes nucleic acid, ma ke nakinaki ʻana i nā molekala ʻike i hoʻopili ʻia e avidin. Hoʻohana pinepine ʻia kēia ʻano hana ma ELISA, immunohistochemistry, a me nā hoʻāʻo hybridization nucleic acid.

Ka Hoʻouna ʻana i nā Lāʻau Lapaʻau i Kuhikuhi ʻia: Hiki ke hoʻohana ʻia ʻo Avidin ma ke ʻano he mole lawe e hoʻouna i nā lāʻau lapaʻau a i ʻole nā ​​​​mea lapaʻau e pili pono ana i nā pūnaewele a i ʻole nā ​​​​ʻiʻo e hōʻike ana i ka biotin receptor. Ma ka hoʻohui ʻana i ka biotin i nā lāʻau lapaʻau a i ʻole nā ​​​​nanoparticles a me ka hoʻopili ʻana iā lākou i avidin, hiki ke hana ʻia nā ʻōnaehana hoʻouna lāʻau lapaʻau i kuhikuhi ʻia.

Nā ʻenehana Biology Molecular: Hoʻohana nui ʻia nā ʻōnaehana i hoʻokumu ʻia e Avidin i ka noiʻi biology molecular. No ka laʻana, ʻo streptavidin, nona ka pilina hoʻopaʻa like me ka biotin e like me avidin, hoʻohana ʻia i nā ʻenehana e like me ka DNA sequencing, PCR, a me ka in situ hybridization, kahi i hui pū ʻia ai me nā probes biotinylated a i ʻole nā ​​​​primers.

Nā Kiʻi a me nā Hana Nānā: Hiki ke hoʻopaʻa inoa ʻia ʻo Avidin me nā radioisotopes, nā dyes fluorescent, a i ʻole nā ​​nanoparticles, e lilo ia i mea hana waiwai nui i nā ʻano hana kiʻi e like me ka positron emission tomography (PET), fluorescence microscopy, a me ka magnetic resonance imaging (MRI). Eia kekahi, hoʻohana ʻia nā ʻōnaehana e pili ana i ka avidin i nā hoʻokolohua diagnostic, e like me nā immunoassays, e ʻike i ke alo o nā biomarkers kikoʻī.