BCIP Na) 5-Bromo-4-chloro-3-indolyl phosphate disodium paʻakai CAS:102185-33-1

Hopena:

He hui kala ʻole ʻo BCIP i hoʻololi ʻia i loko o kahi precipitate polū-poni ma muli o ka hana a ka alkaline phosphatase. ʻO kēia hoʻololi kala e ʻae ai i ka ʻike maka a me ka helu ʻana o ka hana a ka enzyme.

Nā noi:

Nā hoʻāʻo enzyme: Hoʻohana ʻia ʻo BCIP i nā hoʻāʻo biochemical like ʻole e ana i ka hana o ka alkaline phosphatase. Hoʻokomo pū kēia i ka ʻike ʻana i ka hana phosphatase i loko o nā lysates cell, nā enzymes i hoʻomaʻemaʻe ʻia, a i ʻole nā ​​​​hoʻāʻo in vitro.

Immunohistochemistry: Hiki ke hoʻohana ʻia ʻo BCIP e ʻike i nā antibodies i hōʻailona ʻia me ka alkaline phosphatase i nā ʻāpana ʻiʻo a i ʻole ma nā ʻili o ke kelepona. Hoʻohana pinepine ʻia kēia ʻano hana i nā protocols immunostaining.

Nā ʻano hana blotting: Hoʻohana ʻia ʻo BCIP ma Western blotting, Southern blotting, a me Northern blotting e ʻike i nā protein kikoʻī a i ʻole nā ​​​​​​waikawa nucleic i lepili ʻia me nā probes alkaline phosphatase-conjugated.

Hoʻohuihui in situ: Hiki ke hoʻohana ʻia ʻo BCIP e nānā i nā ʻano hōʻike gene i loko o nā ʻiʻo a i ʻole nā ​​​​​​pūnaewele ma o nā ʻano hybridization in situ.

Nā hoʻāʻo diagnostic: Hoʻohana ʻia ʻo BCIP ma ke ʻano he substrate i loko o nā pahu diagnostic like ʻole no ka ʻike ʻana i ka alkaline phosphatase. Hoʻohana ʻia kēia mau pahu i nā keʻena hoʻokolohua lapaʻau no ka ʻike ʻana a me ka nānā ʻana i nā maʻi.