BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine paʻakai CAS:6578-06-9

ʻO ka blotting Komohana: Hoʻohana ʻia ʻo BCIP e ʻike a nānā i nā protein i hoʻoili ʻia ma luna o nā membrane ma hope o ka electrophoresis gel. Hoʻohana ʻia nā antibodies lua i hoʻohui ʻia me ka alkaline phosphatase e hoʻopaʻa i nā antibodies mua, a ʻo ka incubation ma hope me BCIP e hopena i ka hoʻomohala ʻana o kahi precipitate polū ma kahi o nā hui protein.

Immunohistochemistry: Hoʻohana ʻia ʻo BCIP i nā ʻano hana hoʻoluʻu immunohistochemical e ʻike i ke alo o nā protein kikoʻī i loko o nā ʻiʻo. Hoʻohana ʻia nā antibodies lua i hoʻohui ʻia me ka alkaline phosphatase e hoʻopaʻa i nā antibodies mua, a hoʻopuka ka mālama ʻana o BCIP i kahi kala polū i ke alo o ka enzyme, e hōʻike ana i ka localization protein i loko o ka hāpana ʻiʻo.

Hoʻohuihui ʻia ma kahi: Hoʻohana ʻia ʻo BCIP no ka ʻike ʻana i nā moʻo waikawa nucleic i loko o nā ʻiʻo paʻa a i ʻole nā ​​​​​​kelepona. Hoʻopaʻa ʻia nā probes hybridization me ka alkaline phosphatase, a ma ka hoʻohui ʻana o BCIP, hōʻike ʻia ke alo o ka mRNA a i ʻole DNA i manaʻo ʻia e ka hoʻomohala ʻana o kahi kala polū.