BCIP-Toluidine)5-Bromo-4-chloro-3-indolylphosphate-p-toluidine paʻakai CAS:6578-06-9

ʻO BCIP-Toluidine, i ʻike ʻia hoʻi ʻo 5-Bromo-4-chloro-3-indolylphosphate-p-toluidine salt, he hui kemika i hoʻohana pinepine ʻia i ka biology molecular a me ka noiʻi biochemistry. Hoʻohana nui ʻia ia ma ke ʻano he substrate i nā enzyme-linked immunosorbent assays (ELISAs) a i ʻole nā ​​​​​​ʻano hana hoʻoluʻu immunohistochemical e ʻike i ka hana alkaline phosphatase.

Ke hoʻokaʻawale ʻia ʻo BCIP-Toluidine e ka alkaline phosphatase, loaʻa iā ia kahi hopena hoʻololi kala, e hana ana i kahi precipitate polū-poni. ʻAe kēia hopena i ka nānā ʻana a me ka helu ʻana o ka hana alkaline phosphatase i loko o nā laʻana. Hiki ke ana ʻia ka ikaika o ke kala ma ke ʻano spectrophotometric a pili pono i ka hana enzyme.

ʻO ka hoʻohana ʻana o BCIP-Toluidine ma ke ʻano he substrate he mea maʻamau ia i nā noi e pili ana i ka lepili DNA a i ʻole RNA, e like me ka in situ hybridization (ISH) a i ʻole Southern and Northern blotting. Hāʻawi ia i kahi ala hilinaʻi a koʻikoʻi no ka ʻike ʻana i ke alo o nā waikawa nucleic i manaʻo ʻia i loko o nā laʻana.