ʻO Concanavalin A CAS: 11028-71-0 Kumukūʻai Mea Hana

Hoʻohuihui Kelepona: ʻO kekahi o nā hopena koʻikoʻi o Con A ʻo ia kona hiki ke hoʻoulu i ka agglutination (clumping) o nā kelepona. Ua lilo kēia waiwai i mea pono no ke aʻo ʻana i ka hoʻopili ʻana o nā kelepona a me nā pilina o ka ʻili o nā kelepona.

Hoʻokaʻawale ʻana o Glycoprotein: Hoʻopaʻa pono ʻo Con A i nā glycoproteins e loaʻa ana nā koena mannose a me nā glucose. Ua hoʻohana ʻia kēia waiwai e hoʻokaʻawale a hoʻomaʻemaʻe i nā glycoproteins mai nā hui paʻakikī, e like me nā wai olaola a i ʻole nā ​​lysates cell, ma ka hoʻohana ʻana i ka chromatography affinity Con A.

Ka ʻIke ʻana i nā Glycoproteins: Hiki ke hoʻohana ʻia ʻo Con A e ʻike a helu i nā glycoproteins i loko o nā laʻana. Ma ka hoʻohana ʻana iā Con A i hui pū ʻia me nā lepili fluorescent a i ʻole enzymatic, hiki ke ʻike a ana paha i ka nui o nā glycoproteins i loko o kahi laʻana ma o ka microscopy fluorescence a i ʻole nā ​​​​ʻenehana enzyme-linked immunosorbent assay (ELISA).

Ka Lepili ʻIli Kelepona: Ma muli o kona pili ʻana i nā kō ʻili kelepona, hiki ke hoʻohana ʻia ʻo Con A e lepili i nā ʻano kelepona kikoʻī a i ʻole e aʻo i nā ʻano glycosylation ma nā ʻili kelepona. ʻO Con A i hoʻohui ʻia me nā fluorophores a i ʻole nā ​​​​lepili ʻē aʻe e ʻae i ka nānā ʻana a me ka nānā ʻana i nā kelepona ma o nā ʻano hana e like me ke kahe cytometry a i ʻole ka microscopy fluorescence.

Immunomodulation: Loaʻa iā Con A nā waiwai immunomodulatory a hiki ke hoʻopilikia i ka hana o nā cell immune. Hiki iā ia ke hoʻoulu i ka hoʻoulu ʻana a me ka hoʻonui ʻana o nā lymphocytes T, e alakaʻi ana i ka hana ʻana o nā cytokines a me nā pane immune ʻē aʻe. Ua ʻimi ʻia kēia waiwai i ka immunotherapy a me ka hoʻomohala ʻana i ka lāʻau lapaʻau, kahi i hoʻohana ʻia ai ʻo Con A e hoʻoikaika i nā pane immune a i ʻole e hoʻololi i ka hoʻomanawanui immune.

Mea Hana Noiʻi: He mea hana i hoʻohana nui ʻia ʻo Con A ma nā ʻano like ʻole o ka noiʻi olaola. Ua hoʻohana ʻia ia e aʻo i nā pilina carbohydrate-protein, ka hoʻopili ʻana o nā cell, nā ʻano glycosylation, a me nā pane pale. ʻO kona hiki ke hoʻopaʻa i nā kō kikoʻī e lilo ia i mea waiwai no ke aʻo ʻana a me ka hoʻoponopono ʻana i nā glycoproteins a me nā glycolipids.