DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) CAS:28718-90-3

ʻO DAPI (4,6-Diamidino-2-phenylindole dihydrochloride) kahi kala fluorescent i hoʻohana pinepine ʻia i ka noiʻi olaola no ka nānā ʻana i ka DNA. Hoʻopaʻa ia i ka groove liʻiliʻi o ka DNA kaula pālua a hoʻopuka i ka fluorescence polū ke hoʻomālamalama ʻia me ka mālamalama ultraviolet. Hoʻohana pinepine ʻia ʻo DAPI i ka microscopy fluorescence e hoʻoluʻu i nā nuclei cell, e lilo ia i mea hana waiwai no ke aʻo ʻana i ke ʻano a me ka hoʻonohonoho ʻana o ka DNA i loko o nā cell. Hoʻohana ʻia hoʻi ia i ka cytometry kahe e kālailai i ka ʻike DNA i loko o nā cell pākahi a me nā ʻano hana ʻē aʻe no ka nānā ʻana a me ka helu ʻana o ka DNA. ʻO ke kiʻekiʻe o ka pilina o DAPI no ka DNA a me kona mau waiwai fluorescent kikoʻī e lilo ia i mea hana maʻalahi a hoʻohana nui ʻia i ka noiʻi biology molekala a me ke cell.