Disodium 4-nitrophenylphosphate CAS:4264-83-9

ʻO ka hopena nui a me ka hoʻohana ʻana o ka disodium 4-nitrophenylphosphate ʻo ia kona hoʻohana ʻia ʻana ma ke ʻano he substrate chromogenic no nā hoʻāʻo enzyme alkaline phosphatase. Ke hoʻomoʻa ʻia kēia hui me ka alkaline phosphatase, hoʻokaʻawale ka enzyme i ka hui phosphate mai ka disodium 4-nitrophenylphosphate, e hopena ana i ka hoʻokumu ʻia ʻana o 4-nitrophenol.

ʻO ka 4-nitrophenol i hana ʻia he hui melemele ia e hiki ke ʻike ʻia ma ke ʻano spectrophotometrically ma 405 nm. Hoʻohana ʻia kēia hoʻololi kala e ana i ka hana o ka alkaline phosphatase i loko o nā laʻana, e like me nā wai olaola a i ʻole nā ​​lysates cell.

ʻO ka hoʻopili ʻana o ka disodium 4-nitrophenylphosphate i nā hoʻāʻo enzyme e hāʻawi i kahi ala kūpono no ke ana ʻana i ka hana alkaline phosphatase. Pili kēia enzyme i nā kaʻina hana olaola like ʻole a hoʻohana pinepine ʻia ma ke ʻano he māka no ka hoʻokaʻawale ʻana o nā ʻiʻo, ka hana o ke ake, a me ka metabolism o ka iwi.

Eia kekahi, hiki ke hoʻohana ʻia ka disodium 4-nitrophenylphosphate ma ke ʻano he substrate no nā phosphatases ʻē aʻe, e like me ka acid phosphatase a i ʻole nā ​​​​protein phosphatases, ma muli o ka hoʻonohonoho hoʻokolohua a me ka pahuhopu noiʻi.