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PNPG CAS:3150-24-1 Kumukūʻai Mea Hana
ʻO PNPG, a i ʻole p-nitrophenyl β-D-glucopyranoside, he substrate mole liʻiliʻi i hoʻohana pinepine ʻia i nā hoʻokolohua biochemical e ana i ka hana o nā enzymes glucosidase.ʻAʻole waihoʻoluʻu a me ka fluorescent, akā ma luna o ka hydrolysis e glucosidase, ua hoʻololi ʻia i p-nitrophenol, nona ka waihoʻoluʻu melemele a hiki ke ʻike maʻalahi i ka spectrophotometrically.
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ONPG CAS:369-07-3 Kumukūʻai Mea Hana
ʻO ka O-nitrophenyl-β-D-galactopyranoside (ONPG) he substrate synthetic i hoʻohana ʻia i ka biochemical a me ka molecular biology assays e ana i ka hana o ka enzyme β-galactosidase.Hoʻohana maʻamau ia i nā hoʻāʻo e ʻike i ka hōʻike gene i nā ʻōnaehana bacteria, e like me Escherichia coli.ONPG he hui kala ʻole i hoʻokaʻawale ʻia e β-galactosidase, ka hopena i ka hoʻokuʻu ʻana i kahi hui melemele, o-nitrophenol.Hiki ke ana ʻia ka waihoʻoluʻu melemele ma ke ʻano spectrophotometrically, e hāʻawi ana i ke ana pololei ʻole o ka hana enzyme. ʻO ka assay e hoʻohana ana i ka ONPG ʻike pinepine ʻia ʻo ka ONPG assay a he ʻano hoʻohana nui ʻia i ka noiʻi biology molecular e loiloi i nā pae hōʻike o nā genes i mālama ʻia e ka lac operon i loko o nā pūpū bacteria.
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Nitrotetrazolium Blue Chloride CAS:298-83-9
ʻO ka Nitrotetrazolium Blue Chloride (NBT) kahi hōʻailona redox i hoʻohana mau ʻia i nā hoʻokolohua biological a me biochemical assays.He pauda melemele ʻeleʻele e lilo i polū ke hoʻemi ʻia, pono ia no ka ʻike ʻana i ka hele ʻana o kekahi mau enzymes a me ka hana metabolic.
Hoʻopili ka NBT me nā mea lawe electron a me nā enzymes e like me dehydrogenases, i komo i loko o nā kaʻina hana kelepona.Ke hoʻemi ʻia ka NBT e kēia mau enzymes, hoʻokumu ia i ka formazan precipitate, e ʻae ai i ka ʻike ʻike a i ʻole spectrophotometric.
Hoʻohana maʻamau ʻia kēia reagent i nā hoʻokolohua e like me ka hōʻike hōʻemi NBT, kahi e hoʻohana ʻia ai e loiloi i ka hana o ka hanu ʻana o nā cell immune.Hiki ke hoʻohana ʻia e aʻo i nā hana enzyme a me nā ala metabolic i ka noiʻi e pili ana i ke koʻikoʻi oxidative, cell viability, a me nā cell differentiation.
Ua loaʻa iā NBT nā noi ma nā ʻano like ʻole, me ka microbiology, immunology, a me ka cell biology.He mea maʻalahi, paʻa, a maʻalahi hoʻi e hoʻohana, e lilo ia i koho kaulana no nā protocols hoʻokolohua.
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Neocuproine CAS: 484-11-7 Kumukūʻai Mea Hana
ʻO ka Neocuproine kahi mea chelating i hoʻohana mau ʻia i nā noi like ʻole, me ka kemika analytical a me nā lāʻau lapaʻau.Loaʻa iā ia kahi pilina ikaika no nā ion keleawe a hana i nā paʻakikī paʻa me lākou.Hoʻohana kēia waiwai i ka neocuproine no ka ʻike ʻana a me ka helu ʻana i ke keleawe i nā hāʻina a i ʻole nā laʻana.Hoʻohui ʻia, ua aʻo ʻia ka neocuproine no kāna mau noi therapeutic hiki, ʻoi loa i ka mālama ʻana i ka maʻi kanesa a me nā maʻi neurodegenerative.
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IPTG CAS:367-93-1 Kumukūʻai Mea Hana
ʻO Isopropyl β-D-1-thiogalactopyranoside (IPTG) kahi analog synthetic o ka lactose i hoʻohana mau ʻia i ka noiʻi biology molecular a me nā noi biotechnology.Hoʻohana mua ʻia ʻo IPTG e hoʻowalewale i ka hōʻike ʻana o nā genes i loko o nā ʻōnaehana bacteria, kahi e lawelawe ai ma ke ʻano he molecular trigger e hoʻomaka i ka transcription o nā genes target.
Ke hoʻohui ʻia i ka ulu ulu, lawe ʻia ka IPTG e ka bacteria a hiki ke hoʻopaʻa i ka protein repressor lac, e pale ana iā ia mai ka pale ʻana i ka hana o ka lac operon.ʻO ka lac operon he pūʻulu o nā genes i komo i ka lactose metabolism, a i ka wā i wehe ʻia ai ka protein repressor, hōʻike ʻia nā genes.
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HATU CAS:148893-10-1 Kumukūʻai Mea Hana
HATU (1- [bis (dimethylamino) methylene]-1H-1,2,3-triazolo [4,5-b] pyridinium 3-oxid hexafluorophosphate) he mea hoʻohui maʻamau i hoʻohana ʻia i ka peptide synthesis a me ka kemika organik.
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D-fucose CAS:3615-37-0 Kumukūʻai Mea Hana
ʻO D-fucose kahi monosaccharide, ʻo ia hoʻi he kōpaʻa kalapona ʻeono, no ka hui o nā kō maʻalahi i kapa ʻia hexoses.He isomer o ka glucose, ʻokoʻa i ka hoʻonohonoho ʻana o hoʻokahi pūʻulu hydroxyl.
Loaʻa maoli ʻia ka D-fucose i nā mea ola like ʻole, me ka bacteria, fungi, nā mea kanu, a me nā holoholona.He kuleana koʻikoʻi ia i nā kaʻina hana olaola, e like me ka hōʻailona cell, cell adhesion, a me ka glycoprotein synthesis.He ʻāpana ia o nā glycolipids, glycoproteins, a me nā proteoglycans, e pili ana i ka kamaʻilio cell-to-cell a me ka ʻike.
I loko o ke kanaka, ua komo pū ʻo D-fucose i ka biosynthesis o nā hale glycan koʻikoʻi, e like me nā antigens Lewis a me nā antigens hui koko, i loaʻa nā hopena i ka hoʻohālikelike ʻana i ke koko a me ka maʻi maʻi.
Hiki ke loaʻa ka D-fucose mai nā kumu like ʻole, me ka limu, nā mea kanu, a me ka fermentation microbial.Hoʻohana ʻia ia i ka noiʻi a me nā noi biomedical, a me ka hana ʻana i kekahi mau lāʻau lapaʻau a me nā pūhui therapeutic.
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DDT CAS:3483-12-3 Kumukūʻai Mea Hana
ʻO DL-Dithiothreitol, i kapa ʻia ʻo DTT, he mea hoʻohaʻahaʻa i hoʻohana mau ʻia i ka noiʻi biochemical a me ka molecular biology.He mole liʻiliʻi ia me kahi hui thiol (ʻokoʻa i ka sulfur) ma kēlā me kēia ʻaoʻao.
Hoʻohana pinepine ʻia ʻo DTT e wāwahi i nā paʻa disulfide i loko o nā protein, e kōkua ana i ka wehe ʻana a i ʻole ka denature iā lākou.He mea koʻikoʻi kēia hōʻemi o nā paʻa disulfide i nā kaʻina hana like ʻole e like me ka hoʻomaʻemaʻe ʻana i ka protein, ka electrophoresis gel, a me nā haʻawina hoʻolālā protein.Hiki ke ho'ohana 'ia ka DTT no ka pale 'ana i nā hui thiol a pale i ka oxidation i nā ka'ina hana ho'okolohua.
Hoʻohui pinepine ʻia ʻo DTT i nā hoʻonā hoʻokolohua ma nā haʻahaʻa liʻiliʻi, a hilinaʻi kāna hana i ka loaʻa ʻana o ka oxygen.He mea nui ka mālama pono ʻana i ka DTT no ka mea ua maʻalahi ia i ka ea, ka wela, a me ka makū, hiki ke hōʻemi i kona pono.
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D-(+)-Galactose CAS:59-23-4 Kumukūʻai Mea Hana
ʻO D-(+)-Galactose he kōpaʻa monosaccharide a he mea koʻikoʻi o nā kaʻina olaola he nui.He kō maʻamau ia i loaʻa i nā meaʻai he nui, e like me nā huaʻai, nā meaʻai waiū, a me nā mea kanu.
Hoʻopili pinepine ʻia ʻo Galactose i ke kino ma o ke ʻano o nā hopena enzymatic.He hana koʻikoʻi ia i ke kamaʻilio kelepona, ka hana ikehu, a me ka biosynthesis o nā molekole koʻikoʻi e like me glycolipids, glycoproteins, a me ka lactose.
Ma ke ʻano o kāna mau noi, hoʻohana mau ʻia ʻo D-(+)-Galactose i ka microbiology a me ka biotechnology ma ke ʻano he kumu kalapona i ka media moʻomeheu no ka ulu ʻana o nā microorganisms like ʻole.Hoʻohana ʻia ia i ka hana ʻana i nā pūhui bioactive like ʻole, nā lāʻau lapaʻau, a me nā huahana meaʻai.Hoʻohui ʻia, hoʻohana pinepine ʻia ʻo ia ma ke ʻano he lāʻau diagnostic lāʻau lapaʻau, ʻoi loa i nā hoʻokolohua no ka loiloi ʻana i ka hana o ka ate a me ka ʻike ʻana i nā maʻi genetic e pili ana i ka galactose metabolism.
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beta-D-Galactose pentaacetate CAS:4163-60-4
ʻO ka Beta-D-Galactose pentaacetate he mea hoʻohui kemika i loaʻa mai ka galactose, he kōpaʻa monosaccharide.Hoʻokumu ʻia ia ma ka acetylating i kēlā me kēia pūʻulu hydroxyl o ka mole galactose me nā hui acetyl ʻelima.
Hoʻohana pinepine ʻia kēia pūhui ma ke ʻano he mea pale no ka galactose i nā ʻano hana kemika a me nā kaʻina hana synthetic.Kōkua ke ʻano pentaacetate i ka hoʻopaʻa ʻana i ka galactose a pale i nā hopena makemake ʻole a i ʻole nā hoʻololi i ka wā o ka hopena.
Eia hou, hiki ke hoʻohana ʻia kēia pūhui ma ke ʻano he precursor no ka synthesis o nā derivative galactose ʻē aʻe.Hiki ke hoʻoneʻe ʻia nā hui acetyl no ka loaʻa ʻana o nā derivatives galactose like ʻole me nā pūʻulu hana kikoʻī.
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5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium paʻakai CAS:129541-41-9
ʻO 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium paʻakai he mea hoʻohui kemika i hoʻohana mau ʻia i ka noiʻi ʻana a me nā diagnostics.Ua kapa pinepine ʻia ʻo X-Gluc a hoʻohana nui ʻia ma ke ʻano he substrate no ka ʻike ʻana i ka hana enzyme beta-glucuronidase.
Ke loaʻa ka beta-glucuronidase, hoʻokaʻawale ia i ka paʻa glucuronide i X-Gluc, ka hopena i ka hoʻokuʻu ʻana o kahi wai uliuli i kapa ʻia ʻo 5-bromo-4-chloro-3-indolyl.Hoʻohana mau ʻia kēia pane no ka ʻike maka a i ʻole spectrophotometrically i ka hōʻike ʻana o ka enzyme beta-glucuronidase i loko o nā pūnaewele a i ʻole nāʻiʻo.
ʻO ke ʻano paʻakai sodium o X-Gluc e hoʻomaikaʻi i kona solubility i loko o nā wai wai, e hoʻomaʻamaʻa i kona hoʻohana ʻana i nā hoʻokolohua ʻoihana.Hoʻohana nui ʻia ʻo X-Gluc i ka noiʻi biology molecular e aʻo ai i ka ʻōlelo gene, ka hana hoʻolaha, a me nā hōʻike gene reporter.Hiki ke hoʻohana ʻia no ka ʻike ʻana i ka hiki ʻana o nā mea hana beta-glucuronidase, e like me kekahi mau bacteria, i nā haʻawina microbiological.
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4-Nitrophenyl-beta-D-xylopyranoside CAS:2001-96-9
ʻO 4-Nitrophenyl-beta-D-xylopyranoside he substrate chromogenic i hoʻohana ʻia i nā hoʻokolohua enzymatic e ʻike a ana i ka hana o nā enzyme i kapa ʻia beta-xylosidases.
