Nitrotetrazolium Blue Chloride CAS: 298-83-9

ʻO Nitrotetrazolium Blue Chloride (NBT) kahi hōʻailona redox i hoʻohana pinepine ʻia i nā hoʻāʻo biological a me biochemical. He pauka melemele palupalu ia e lilo i polū ke hoʻemi ʻia, e lilo ia i mea pono no ka ʻike ʻana i ke alo o kekahi mau enzymes a me ka hana metabolic.

ʻO ka hopena nui o NBT ka hoʻokumu ʻia ʻana o kahi formazan polū i hoʻemi ʻia e kekahi mau enzymes. ʻO kēia hoʻololi kala e ʻae ai i ka ʻike ʻana i ka hana enzyme ma ka ʻike maka a i ʻole ka spectrophotometric.

He nui nā noi a NBT i ka noiʻi a me ka diagnostics. Eia kekahi o kāna mau hoʻohana nui:

Nā hoʻāʻo hana enzyme: Hiki ke hoʻohana ʻia ʻo NBT e ana i ka hana o nā dehydrogenases, ka mea e pili ana i nā kaʻina hana e like me ka hanu ʻana o ke kelepona a me ka metabolism. Ma ka nānā ʻana i ka hōʻemi ʻana o NBT i formazan, hiki i nā mea noiʻi ke loiloi i ka hana o kēia mau enzymes.

Loiloi o ka hana o nā pūnaewele pale: Hoʻohana pinepine ʻia ka NBT i ka hoʻāʻo hōʻemi NBT e loiloi i ka hana pahū hanu o nā pūnaewele pale, ʻoiai hoʻi nā phagocytes. Ana ka hoʻāʻo i ka hiki o kēia mau pūnaewele ke hana i nā ʻano oxygen reactive, hiki ke hōʻemi i ka NBT a hana i kahi precipitate polū.

Noiʻi Microbiology: Hoʻohana ʻia ʻo NBT i ka microbiology e aʻo i ka metabolism microbial a loiloi i ka hana o nā enzymes kikoʻī. No ka laʻana, ua hoʻohana ʻia e ʻike i nā bacteria nitrate reductases a i ʻole nā ​​​​bacteria formazan-forming.

Nā haʻawina ola o nā kelepona: ʻO ka hōʻemi ʻana o NBT e hiki ai i nā mea noiʻi ke loiloi i ka hana metabolic a me ke ola o nā kelepona. Ma ka helu ʻana i ka ikaika o ka huahana formazan polū, hiki ke hoʻoholo i ka helu o nā kelepona ola i loko o kahi hāpana i hāʻawi ʻia.