ʻO Phenylgalactoside, i kapa ʻia ʻo p-nitrophenyl β-D-galactopyranoside (pNPG), he substrate synthetic i hoʻohana pinepine ʻia i nā hoʻokolohua biochemical a me ka molecular biology.Hoʻohana maʻamau ia e ʻike a ana i ka hana o ka enzyme β-galactosidase.
Ke hoʻoheheʻe ʻia ka phenylgalactoside e β-galactosidase, hoʻokuʻu ʻo ia i ka p-nitrophenol, ʻo ia ka hui melemele.Hiki ke ana ʻia ka hoʻokuʻu ʻia ʻana o ka p-nitrophenol me ka hoʻohana ʻana i kahi spectrophotometer, no ka mea, hiki ke ʻike ʻia ke komo ʻana o ka p-nitrophenol ma kahi hawewe o 405 nm.