ʻO Phenylgalactoside CAS:2818-58-8

Hopena ma ka hana enzyme: Hoʻohana pinepine ʻia ka Phenylgalactoside e ana i ka hana o ka enzyme β-galactosidase. Ke hydrolyzed ʻia ka phenylgalactoside e β-galactosidase, hoʻokuʻu ia i ka p-nitrophenol. Hiki ke ana ʻia ka hōʻiliʻili ʻana o ka p-nitrophenol ma ke ʻano quantitative, e hāʻawi ana i ka ʻike i ka hana o β-galactosidase. Hoʻohana ʻia kēia hopena i nā noi e like me nā hoʻāʻo enzyme a me nā ʻōnaehana nānā.

Ka loiloi ʻana i ka hōʻike ʻana o ka gene: Hoʻohana pinepine ʻia ka Phenylgalactoside ma ke ʻano he substrate i nā hoʻokolohua biology molekala e aʻo ai i ka hōʻike ʻana o ka gene. ʻO ka gene lacZ, ka mea e hoʻopili ana i ka β-galactosidase, ua hoʻohui pinepine ʻia me nā kaʻina hoʻoponopono o nā genes ʻē aʻe o ka hoihoi. ʻO ka hōʻike ʻana o ka gene lacZ a me ka hydrolysis o phenylgalactoside e β-galactosidase hiki ke hōʻike i ke ʻano hōʻike a me ka pae o ka gene target e aʻo ʻia nei.

Nā ʻōnaehana nānā: ʻO Phenylgalactoside kahi ʻāpana koʻikoʻi o nā ʻōnaehana nānā e hoʻohana ana i ka hana β-galactosidase. ʻO kahi laʻana i ʻike nui ʻia ʻo ke ʻano nānā polū-keʻokeʻo, kahi i hoʻohana ʻia e ʻike i nā cell recombinant a i ʻole nā ​​​​​​cell i hoʻololi ʻia i nā hoʻokolohua biology molecular. ʻO nā kolone i lawe pono i ka DNA recombinant a i ʻole i hana ʻia ka recombination genetic e hōʻike i ka β-galactosidase, e alakaʻi ana i ka hydrolysis o phenylgalactoside a me ka hoʻokumu ʻana o kahi kala polū.

Hoʻomaʻemaʻe protein: I kekahi mau hihia, hiki ke hoʻohana ʻia ka phenylgalactoside ma ke ʻano he ligand no ka affinity chromatography e hoʻomaʻemaʻe i nā protein e hoʻopaʻa pono ʻia a i ʻole e hoʻāla ʻia e β-galactosidase. Loaʻa paha i ka protein hoihoi kahi lepili affinity a i ʻole kahi lepili fusion e loaʻa ana kahi kikowaena hoʻopaʻa β-galactosidase. Ma ka hoʻouna ʻana i ka hui protein ma o kahi kolamu me ka phenylgalactoside i hoʻopaʻa ʻia, hiki ke mālama koho ʻia ka protein i makemake ʻia a ma hope e eluted.