ʻO Fluorescein Isothiocyante CAS: 3326-32-7

Nā waiwai fluorescence: Loaʻa iā FITC kahi hua quantum kiʻekiʻe a hoʻopuka i ka fluorescence ʻōmaʻomaʻo ʻālohilohi i ka wā e hoʻonāukiuki ʻia ai ma kahi o 488 nm. ʻO kona mau waiwai fluorescence e kūpono ia no ka ʻike ʻana me ka hoʻohana ʻana i nā ʻano hana kiʻi like ʻole, e like me ka microscopy fluorescence, flow cytometry, a me ka spectroscopy fluorescence.

Hoʻopaʻa Covalent: Loaʻa i ka FITC kahi hui isothiocyanate e hiki ai iā ia ke hoʻopaʻa covalently i nā hui amino i loaʻa i nā protein, antibodies, a me nā biomolecules ʻē aʻe. Hōʻoia kēia hoʻopaʻa covalent i ka lepili paʻa a kikoʻī o nā molekala target, e hiki ai iā lākou ke ʻike a me ka nānā ʻana.

Immunofluorescence: Hoʻohana pinepine ʻia nā antibodies i hōʻailona ʻia e FITC i nā hoʻāʻo immunofluorescence e ʻike i nā antigens a i ʻole nā ​​​​protein kikoʻī i loko o nā cell a me nā ʻiʻo. ʻO ka fluorescence ʻōmaʻomaʻo ʻālohilohi i hoʻopuka ʻia e FITC e hiki ai i nā mea noiʻi ke ʻike a nānā i ka hoʻolaha ʻana o nā molekala i manaʻo ʻia i loko o nā laʻana olaola.

ʻO ka cytometry kahe: Hoʻohana nui ʻia nā antibodies i hōʻailona ʻia ʻo FITC a i ʻole nā ​​biomolecules fluorochrome-conjugated ʻē aʻe i ka cytometry kahe e kālailai a hoʻokaʻawale i nā cell ma muli o kā lākou mau māka ʻili a i ʻole nā ​​​​​​ʻāpana intracellular. Hoʻohālikelike ʻo FITC me ka laser 488 nm i hoʻohana pinepine ʻia i nā mea hana cytometry kahe.

Nā haʻawina pilina protein: Hiki ke hoʻohana ʻia ʻo FITC i nā haʻawina pilina protein-protein e loiloi i ka nakinaki ʻana a me ka pilina o nā protein. Ma ka lepili ʻana i nā protein kikoʻī me FITC, hiki i nā mea noiʻi ke kālailai i kā lākou pilina me ka hoʻohana ʻana i nā ʻano hana e like me ka fluorescence resonance energy transfer (FRET) a i ʻole ka fluorescence polarization.

Ka hahai ʻana i ka biomolecule: Hiki ke hoʻohana ʻia ʻo FITC e hahai a hahai i ka neʻe ʻana o nā biomolecules i loko o nā cell ola a i ʻole nā ​​​​ʻiʻo. Ma ka lepili ʻana i nā molekala hoihoi me FITC, hiki i nā mea noiʻi ke aʻo i ko lākou localization, dynamics, a me nā ʻano trafficking i ka manawa maoli me ka hoʻohana ʻana i ka microscopy fluorescence.

Nā noi diagnostics: Hoʻohana ʻia nā probes a i ʻole nā ​​antibodies i hōʻailona ʻia e FITC i nā diagnostics no nā noi like ʻole, me ka ʻike ʻana i nā antigens kikoʻī, nā mea hoʻolaha maʻi, a i ʻole nā ​​biomarkers i nā laʻana maʻi. Hāʻawi kēia mau probes fluorescent i ka ʻike koʻikoʻi a kikoʻī, e ʻae ana i ka ʻike wikiwiki a pololei.