FLUORESCEIN MONO-BETA-D-GALACTOPYRANOSIDE CAS:102286-67-9

ʻO ka Fluorescein mono-beta-D-galactopyranoside (FMG) kahi molekala i hoʻohana pinepine ʻia i ka noiʻi olaola ma ke ʻano he substrate e ʻike ai i ke alo a me ka hana o ka enzyme beta-galactosidase. ʻO ka FMG kahi hua o ka lactose kō a ua hui pū ʻia me kahi molekala fluorescein.

ʻO ka hopena nui o FMG, ʻo ia ka hydrolyzed kūikawā ʻia e ka beta-galactosidase, kahi enzyme e wāwahi i ka lactose i galactose a me ka glucose. ʻO kēia hydrolysis enzymatic o FMG ke alakaʻi i ka hoʻokuʻu ʻia ʻana o fluorescein, kahi e hoʻopuka ai i kahi hōʻailona fluorescence ikaika.

ʻO ka hoʻohana nui ʻana o FMG ka ʻike ʻana a me ke ana ʻana i ka hana beta-galactosidase i nā laʻana like ʻole. Loaʻa kēia enzyme i loko o nā meaola he nui, me nā bacteria a me nā cell mammalian, a hiki i kāna hana ke hōʻike i nā kaʻina hana cellular like ʻole a me nā ala metabolic.

Ma ka hoʻohana ʻana iā FMG ma ke ʻano he substrate, hiki ke ana ʻia ka hana beta-galactosidase ma ka nānā ʻana i ka fluorescence i hoʻopuka ʻia e ka fluorescein i hoʻokuʻu ʻia. Hiki ke hana ʻia kēia ana ma nā ʻano hoʻonohonoho hoʻokolohua like ʻole, me nā hoʻāʻo in vitro a me nā haʻawina kiʻi cell ola.

Eia kekahi, hiki ke hoʻohana ʻia ʻo FMG ma ke ʻano he mea hana e aʻo ai i ka hoʻolaha ʻana a me ka localization o ka beta-galactosidase i loko o nā cell. Ma ka hoʻohana ʻana i nā ʻano hana microscopy fluorescent, hiki i nā mea noiʻi ke ʻike i ka fluorescence i hoʻopuka ʻia e FMG ma luna o ka hydrolysis, e ʻae ana iā lākou e hahai i ka hana spatial a me ka temporal o ka beta-galactosidase.