ʻO Gly-Pro p-nitroanilide p-toluenesulfonate paʻakai CAS: 65096-46-0

Hopena: Ua hoʻolālā kūikawā ʻia ka paʻakai Gly-Pro p-nitroanilide p-toluenesulfonate e lilo i substrate no nā peptidases proline-specific. Hoʻokaʻawale kēia mau enzymes i ka pilina peptide ma waena o nā glycine (Gly) a me nā koena proline (Pro), e hoʻokuʻu ana i ka p-nitroanilide (pNA) ma ke ʻano he huahana. Hoʻopili ka molekole pNA i ka mālamalama ma kahi nalu kikoʻī, e ʻae ana i ke ana ʻana o ka pNA i hoʻokuʻu ʻia a me ka helu ʻana o ka hana enzyme.

Hoʻohana: Hoʻohana ʻia ka paʻakai Gly-Pro p-nitroanilide p-toluenesulfonate ma nā wahi noiʻi like ʻole, me ka biochemistry, enzymology, a me ka ʻike ʻana i nā lāʻau lapaʻau, no nā noi aʻe:

Nā hoʻāʻo hana enzyme: Hoʻohana nui ʻia e ana i ka hana o nā peptidases proline-specific, me nā dipeptidyl peptidases (DPPs) a me nā prolyl endopeptidases (PEPs).

Nā mea hoʻopaʻa enzyme nānā: Hiki ke hoʻohana ʻia ka paʻakai Gly-Pro p-nitroanilide p-toluenesulfonate e loiloi i ka hiki ke hoʻopaʻa o nā hui a i ʻole nā ​​​​lāʻau lapaʻau ma ka hana peptidase proline-specific. ʻO kēlā me kēia emi ʻana o ka hoʻokuʻu ʻana o pNA e hōʻike ana i ke alo o kahi mea hoʻopaʻa.

Nā haʻawina kinetics enzyme: Ma ka hoʻololi ʻana i ka nui o ka paʻakai Gly-Pro p-nitroanilide p-toluenesulfonate a me ke ana ʻana i ka wikiwiki o ka hoʻokuʻu ʻana o pNA, hiki ke hoʻoholo ʻia nā palena kinetics enzyme e like me Km (Michaelis-Menten constant) a me Vmax (velocity maximum).

Nā noi diagnostics: Hiki ke pili ka hana o nā peptidases proline-specific i nā hoʻokolohua diagnostic no nā maʻi a me nā kūlana like ʻole, e like me nā maʻi a me nā maʻi ʻaʻai. Hiki ke hoʻohana ʻia ka paʻakai Gly-Pro p-nitroanilide p-toluenesulfonate ma ke ʻano he mea hana e loiloi ai i nā pae hana o kēia mau enzymes i nā laʻana maʻi.